PXD003659-2

PXD003659 is an original dataset announced via ProteomeXchange.

Title	Probing the protein inventory of the human pathogen Clostridium difficile
Description	The anaerobic and spore-forming bacterium Clostridium difficile has turned into an intensively studied species which can be attributed to increasing numbers of infections and rising costs for the health care system. This dataset represents a benchmark proteome of reference strain C. difficile 630erm and provides mass spectrometry based details on identified proteins which was generally missing from hitherto published datasets. An elaborate annotation and visualization of the 3764 open reading frames will serve as a valuable base for researchers trying to evaluate results of global expression studies. Furthermore, protein expression of late exponentially growing cells in the complex medium BHI and in C. difficile minimal medium was compared. Whereas abundance of proteins of DNA metabolism, protein synthesis and cell envelope showed no variation, enzymes for the biosynthesis of some vitamins and purine as well as proteins involved in butanoate fermentation differed significantly depending on the growth medium.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:09:06.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Otto
SpeciesList	scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-17 06:32:53	ID requested
1	2016-08-12 03:20:33	announced
⏵ 2	2024-10-22 04:09:07	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: label-free protein quantification,Clostridium difficile, comprehensive peptide library, benchmark proteome

Andreas Otto
contact affiliation	Institute for Microbiology Department of Microbial Proteomics and Mass Spectrometry Ernst-Moritz-Arndt-University Greifswald F.-L.-Jahn-Str. 15 17489 Greifswald phone: ++49 3834 864073
contact email	andreas.otto@uni-greifswald.de
lab head
Andreas Otto
contact affiliation	Institute for Microbiology
contact email	andreas.otto@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD003659

PRIDE project URI

• PRIDE
  1. PXD003659
     1. Label: PRIDE project
     2. Name: Probing the protein inventory of the human pathogen Clostridium difficile