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PXD003659-1

PXD003659 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProbing the protein inventory of the human pathogen Clostridium difficile
DescriptionThe anaerobic and spore-forming bacterium Clostridium difficile has turned into an intensively studied species which can be attributed to increasing numbers of infections and rising costs for the health care system. This dataset represents a benchmark proteome of reference strain C. difficile 630erm and provides mass spectrometry based details on identified proteins which was generally missing from hitherto published datasets. An elaborate annotation and visualization of the 3764 open reading frames will serve as a valuable base for researchers trying to evaluate results of global expression studies. Furthermore, protein expression of late exponentially growing cells in the complex medium BHI and in C. difficile minimal medium was compared. Whereas abundance of proteins of DNA metabolism, protein synthesis and cell envelope showed no variation, enzymes for the biosynthesis of some vitamins and purine as well as proteins involved in butanoate fermentation differed significantly depending on the growth medium.
HostingRepositoryPRIDE
AnnounceDate2016-08-12
AnnouncementXMLSubmission_2016-08-12_03:20:32.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Otto
SpeciesList scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-02-17 06:32:53ID requested
12016-08-12 03:20:33announced
22024-10-22 04:09:07announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Clostridium difficile, benchmark proteome, comprehensive peptide library, label-free protein quantification
Contact List
Andreas Otto
contact affiliationInstitute for Microbiology Department of Microbial Proteomics and Mass Spectrometry Ernst-Moritz-Arndt-University Greifswald F.-L.-Jahn-Str. 15 17489 Greifswald phone: ++49 3834 864073
contact emailandreas.otto@uni-greifswald.de
lab head
Andreas Otto
contact affiliationInstitute for Microbiology
contact emailandreas.otto@uni-greifswald.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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