PXD003009 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome analysis reveals alterations in protein profile upon ectomycorrhiza establishment in oak roots |
Description | Trees establish a symbiotic relationship with specialized soil fungi, called ectomycorrhizae, which is essential for nutrition, growth and health of temperate forest ecosystems. Understanding the mechanisms governing the establishment and functioning of ectomycorrhiza is important because of the role of forests in sequestering CO2 and also to develop ways to optimize tree productivity and sustainability. Here, we investigated the response of an oak species to ectomycorrhiza formation using a two dimensional differential in gel electrophoresis (2D-DIGE) and MALDI-TOF/TOF mass spectrometry proteomics approach. At the root level, changes in the abundance of 34 unique oak proteins were detected and revealed proteins involved in carbon and energy metabolism, protein processing and degradation, response to oxidative stress, lipid metabolism/transport, nitrogen and phosphorous assimilation and cell wall modification. Proteins supporting the importance of the secretory pathway functioning, in particular of the endoplasmic reticulum, during ectomycorrhiza functioning were identified. These proteins were identified as components of the endoplasmic reticulum folding/chaperoning machinery and proteins involved in the ER quality control system. This study constitutes an important contribution for the understanding of the mechanisms underlying the response of plants to ectomycorrhizal symbiosis establishment. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:26:27.255.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joana Martins |
SpeciesList | scientific name: Quercus; NCBI TaxID: 3511; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | 4800 Proteomics Analyzer |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-10-06 01:19:14 | ID requested | |
1 | 2017-07-14 00:03:41 | announced | |
⏵ 2 | 2024-10-22 04:26:35 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1007/s00572-016-0734-z; |
Sebastiana M, Martins J, Figueiredo A, Monteiro F, Sardans J, Pe, ñ, uelas J, Silva A, Roepstorff P, Pais MS, Coelho AV, Oak protein profile alterations upon root colonization by an ectomycorrhizal fungus. Mycorrhiza, 27(2):109-128(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: symbionsis,Roots, protoemics, ectomycorrhizae, 2D-DIGE |
Contact List
Ana Varela Coelho |
contact affiliation | Laboratory of proteomics of non-model organism in Instituto de Tecnologia Quimica e Biologica |
contact email | varela@itqb.unl.pt |
lab head | |
Joana Martins |
contact affiliation | ITQB-UNL |
contact email | joanam@itqb.unl.pt |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD003009
- Label: PRIDE project
- Name: Proteome analysis reveals alterations in protein profile upon ectomycorrhiza establishment in oak roots