PXD003009-1

PXD003009 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis reveals alterations in protein profile upon ectomycorrhiza establishment in oak roots
Description	Trees establish a symbiotic relationship with specialized soil fungi, called ectomycorrhizae, which is essential for nutrition, growth and health of temperate forest ecosystems. Understanding the mechanisms governing the establishment and functioning of ectomycorrhiza is important because of the role of forests in sequestering CO2 and also to develop ways to optimize tree productivity and sustainability. Here, we investigated the response of an oak species to ectomycorrhiza formation using a two dimensional differential in gel electrophoresis (2D-DIGE) and MALDI-TOF/TOF mass spectrometry proteomics approach. At the root level, changes in the abundance of 34 unique oak proteins were detected and revealed proteins involved in carbon and energy metabolism, protein processing and degradation, response to oxidative stress, lipid metabolism/transport, nitrogen and phosphorous assimilation and cell wall modification. Proteins supporting the importance of the secretory pathway functioning, in particular of the endoplasmic reticulum, during ectomycorrhiza functioning were identified. These proteins were identified as components of the endoplasmic reticulum folding/chaperoning machinery and proteins involved in the ER quality control system. This study constitutes an important contribution for the understanding of the mechanisms underlying the response of plants to ectomycorrhizal symbiosis establishment.
HostingRepository	PRIDE
AnnounceDate	2017-07-14
AnnouncementXML	Submission_2017-07-14_00:03:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joana Martins
SpeciesList	scientific name: Quercus; NCBI TaxID: 3511;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2015-10-06 01:19:14	ID requested
⏵ 1	2017-07-14 00:03:41	announced
2	2024-10-22 04:26:35	announced	2024-10-22: Updated project metadata.

Sebastiana M, Martins J, Figueiredo A, Monteiro F, Sardans J, Pe, ñ, uelas J, Silva A, Roepstorff P, Pais MS, Coelho AV, Oak protein profile alterations upon root colonization by an ectomycorrhizal fungus. Mycorrhiza, 27(2):109-128(2017) [pubmed]

curator keyword: Biological

submitter keyword: Roots, ectomycorrhizae,symbionsis, protoemics, 2D-DIGE

Ana Varela Coelho
contact affiliation	Laboratory of proteomics of non-model organism in Instituto de Tecnologia Quimica e Biologica
contact email	varela@itqb.unl.pt
lab head
Joana Martins
contact affiliation	ITQB-UNL
contact email	joanam@itqb.unl.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD003009
     1. Label: PRIDE project
     2. Name: Proteome analysis reveals alterations in protein profile upon ectomycorrhiza establishment in oak roots