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PXD002764-2

PXD002764 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA combined proteomic and targeted analysis unravels new toxic mechanisms for zinc oxide nanoparticles in macrophages
DescriptionThe cellular responses of the J774 macrophage cell line to zinc oxide and zirconium oxide nanoparticles have been studied by a comparative quantitative, protein level based proteomic approach. The most prominent results have been validated by targeted approaches. These approaches have been carried out under culture conditions that stimulate mildly the aryl hydrocarbon receptor, thereby mimicking conditions that can be encountered in vivo in complex environments. The comparative approach with two nanoparticles allows to separate the common responses, which can be attributed to the phagocytosis event per se, from the response specific to each type of nanoparticles. The zinc-specific responses are the most prominent ones and include mitochondrial proteins too, but also signaling molecules such as MyD88, proteins associated with methylglyoxal detoxification (glyoxalase 2, aldose reductase) and deoxyribonucleotide hydrolases. The in cellulo inhibition of GAPDH by zinc was also documented, representing a possible source of methylglyoxal in the cells, leading to an increase in methylglyoxal-modified DNA bases. These observations may be mechanistically associated with the genotoxic effect of zinc and its selective effects on cancer cells
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:48:50.505.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJean-Marc STRUB
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600; Synapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-08-19 01:56:02ID requested
12022-03-01 09:54:22announced
22023-11-14 08:48:51announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Nanoparticles
macrophages
zinc oxide
zirconium dioxide
proteomics
glutathione biosynthesis
phagocytosis
DNA damage
heme oxygenase
methyglyoxal
mitochondria
Contact List
Thierry Rabilloud
contact affiliationCEA Grenoble, iRTSV/CBM, Laboratory of Chemistry and Biology of Metals, Grenoble, France
contact emailthierry.rabilloud@cea.fr
lab head
Jean-Marc STRUB
contact affiliationCNRS
contact emailjmstrub@unistra.fr
dataset submitter
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Dataset FTP location
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