PXD002764 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A combined proteomic and targeted analysis unravels new toxic mechanisms for zinc oxide nanoparticles in macrophages |
| Description | The cellular responses of the J774 macrophage cell line to zinc oxide and zirconium oxide nanoparticles have been studied by a comparative quantitative, protein level based proteomic approach. The most prominent results have been validated by targeted approaches. These approaches have been carried out under culture conditions that stimulate mildly the aryl hydrocarbon receptor, thereby mimicking conditions that can be encountered in vivo in complex environments. The comparative approach with two nanoparticles allows to separate the common responses, which can be attributed to the phagocytosis event per se, from the response specific to each type of nanoparticles. The zinc-specific responses are the most prominent ones and include mitochondrial proteins too, but also signaling molecules such as MyD88, proteins associated with methylglyoxal detoxification (glyoxalase 2, aldose reductase) and deoxyribonucleotide hydrolases. The in cellulo inhibition of GAPDH by zinc was also documented, representing a possible source of methylglyoxal in the cells, leading to an increase in methylglyoxal-modified DNA bases. These observations may be mechanistically associated with the genotoxic effect of zinc and its selective effects on cancer cells |
| HostingRepository | PRIDE |
| AnnounceDate | 2022-03-01 |
| AnnouncementXML | Submission_2022-03-01_09:54:21.914.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jean-Marc STRUB |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | TripleTOF 5600; Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2015-08-19 01:56:02 | ID requested | |
| ⏵ 1 | 2022-03-01 09:54:22 | announced | |
| 2 | 2023-11-14 08:48:51 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
| curator keyword: Biomedical |
| submitter keyword: Nanoparticles |
| macrophages |
| zinc oxide |
| zirconium dioxide |
| proteomics |
| glutathione biosynthesis |
| phagocytosis |
| DNA damage |
| heme oxygenase |
| methyglyoxal |
| mitochondria |
Contact List
| Thierry Rabilloud |
|---|
| contact affiliation | CEA Grenoble, iRTSV/CBM, Laboratory of Chemistry and Biology of Metals, Grenoble, France |
| contact email | thierry.rabilloud@cea.fr |
| lab head | |
| Jean-Marc STRUB |
|---|
| contact affiliation | CNRS |
| contact email | jmstrub@unistra.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD002764 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002764
- Label: PRIDE project
- Name: A combined proteomic and targeted analysis unravels new toxic mechanisms for zinc oxide nanoparticles in macrophages
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