PXD002214-1

PXD002214 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteome Analysis Reveals Increased Content of Basement Membrane Proteins in Arteries from Patients with Type 2 Diabetes and Lower Levels among Metformin Users
Description	We analyzed non-atherosclerotic repair arteries gathered at coronary by-pass operations from 30 patients with type 2 diabetes, as well as from 30 age- and gender-matched non-diabetic individuals. Quantitative proteome analysis was done by iTRAQ-labelling and LC-MS/MS analysis on individual arterial samples. The amounts of the basement membrane (BM) components, alpha-1- and alpha-2- type IV collagen, gamma-1- and beta-2-laminin were significantly increased in patients with diabetes. Moreover, the expressions of basement membrane components and other vascular proteins were significantly lower among metformin users, as compared to non-users. Patients treated with or without metformin had similar levels of HbA1c, cholesterol and blood pressure. In addition, quantitative histomorphometry showed increased area fractions of collagen-stainable material in tunica intima and media among patients with diabetes.
HostingRepository	PRIDE
AnnounceDate	2017-02-20
AnnouncementXML	Submission_2017-02-20_06:06:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hans Christian Beck
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-21 01:22:23	ID requested
⏵ 1	2017-02-20 06:06:55	announced
2	2024-10-22 04:24:38	announced	2024-10-22: Updated project metadata.

Preil SA, Kristensen LP, Beck HC, Jensen PS, Nielsen PS, Steiniche T, Bj, ø, rling-Poulsen M, Larsen MR, Hansen ML, Rasmussen LM, Quantitative Proteome Analysis Reveals Increased Content of Basement Membrane Proteins in Arteries From Patients With Type 2 Diabetes Mellitus and Lower Levels Among Metformin Users. Circ Cardiovasc Genet, 8(5):727-35(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: type 2 diabetes artery proteomics

Hans Christian Beck
contact affiliation	Odense University Hospital, Sdr Boulevard 29, DK-5000 Odense C, Dept Clinical Biochemistry and Pharmacology
contact email	hans.christian.beck@rsyd.dk
lab head
Hans Christian Beck
contact affiliation	Odense University Hospital, Odense, Denmark
contact email	hans.christian.beck@rsyd.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002214
     1. Label: PRIDE project
     2. Name: Quantitative Proteome Analysis Reveals Increased Content of Basement Membrane Proteins in Arteries from Patients with Type 2 Diabetes and Lower Levels among Metformin Users