PXD033711 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TAG-TMTpro, a hyperplexing quantitative approach for high-throughput proteomic studies |
Description | Isobaric labeling is the most widely used multiplexing quantitative approach in proteomic studies, enabling the comparison of up to 18 samples in a single MS analysis. Expanding the multiplexing capacity is of great necessity for high-throughput proteomic studies. Herein, we establish a novel TAG-TMTpro approach by introducing Ala or Gly residues to peptides prior to TMTpro labeling, which is able to triple the quantitative capacity of TMTpro. We systematically evaluated the Boc-Ala-OSu and Boc-Gly-OSu reaction and optimized the conditions for labeling, side-product elimination and Boc deprotection. We validated the identification and quantification performance using E. coli and HeLa cell lysates. We demonstrated that the TAG-TMTpro approach resulted in good identification reproducibility and reliable quantitative accuracy. The TAG-TMTpro is able to triple the multiplexing capacity of TMTpro reagents, and is a versatile quantitative approach for high-throughput proteomic studies. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:11:34.015.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhen Wu |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidoethyl-L-cysteine; glycine residue; monohydroxylated residue; L-alanine residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-05 01:19:05 | ID requested | |
1 | 2022-10-14 07:24:52 | announced | |
⏵ 2 | 2023-11-14 08:11:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wu Z, Shen Y, Zhang X, TAG-TMTpro, a Hyperplexing Quantitative Approach for High-Throughput Proteomic Studies. Anal Chem, 94(37):12565-12569(2022) [pubmed] |
Keyword List
submitter keyword: proteomics, multiplexing quantitative approach,TMTpro labeling |
Contact List
Xumin Zhang |
contact affiliation | State Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China |
contact email | xumin_zhang@fudan.edu.cn |
lab head | |
Zhen Wu |
contact affiliation | State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University |
contact email | wuz16@fudan.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033711
- Label: PRIDE project
- Name: TAG-TMTpro, a hyperplexing quantitative approach for high-throughput proteomic studies