PXD033711-1

PXD033711 is an original dataset announced via ProteomeXchange.

Title	TAG-TMTpro, a hyperplexing quantitative approach for high-throughput proteomic studies
Description	Isobaric labeling is the most widely used multiplexing quantitative approach in proteomic studies, enabling the comparison of up to 18 samples in a single MS analysis. Expanding the multiplexing capacity is of great necessity for high-throughput proteomic studies. Herein, we establish a novel TAG-TMTpro approach by introducing Ala or Gly residues to peptides prior to TMTpro labeling, which is able to triple the quantitative capacity of TMTpro. We systematically evaluated the Boc-Ala-OSu and Boc-Gly-OSu reaction and optimized the conditions for labeling, side-product elimination and Boc deprotection. We validated the identification and quantification performance using E. coli and HeLa cell lysates. We demonstrated that the TAG-TMTpro approach resulted in good identification reproducibility and reliable quantitative accuracy. The TAG-TMTpro is able to triple the multiplexing capacity of TMTpro reagents, and is a versatile quantitative approach for high-throughput proteomic studies.
HostingRepository	PRIDE
AnnounceDate	2022-10-14
AnnouncementXML	Submission_2022-10-14_07:24:51.328.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ZhenWu
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; glycine residue; monohydroxylated residue; L-alanine residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 01:19:05	ID requested
⏵ 1	2022-10-14 07:24:52	announced
2	2023-11-14 08:11:34	announced	2023-11-14: Updated project metadata.

Wu Z, Shen Y, Zhang X, TAG-TMTpro, a Hyperplexing Quantitative Approach for High-Throughput Proteomic Studies. Anal Chem, 94(37):12565-12569(2022) [pubmed]

submitter keyword: proteomics, multiplexing quantitative approach,TMTpro labeling

XuminZhang
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China
contact email	xumin_zhang@fudan.edu.cn
lab head
ZhenWu
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University
contact email	wuz16@fudan.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033711
     1. Label: PRIDE project
     2. Name: TAG-TMTpro, a hyperplexing quantitative approach for high-throughput proteomic studies