PXD027068-1

PXD027068 is an original dataset announced via ProteomeXchange.

Title	Interactome analysis of C. elegans synapses by TurboID-based proximity labeling
Description	Proximity labeling provides a powerful in vivo tool to characterize the proteome of subcellular structures and the interactome of specific proteins. Using the highly active biotin ligase TurboID, we optimize a proximity labeling protocol for C. elegans. We use this protocol to characterise the proteomes of the worm’s gut, muscle, skin, and nervous system. We express TurboID exclusively in the pair of AFD neurons and show we can identify known and previously unknown proteins expressed selectively in AFD. We knock TurboID into the endogenous elks-1 gene, which encodes a presynaptic active zone protein. We identify many known ELKS-1 interacting proteins as well as previously uncharacterised synaptic proteins. Versatile vectors, and the inherent advantage of C. elegans for biochemistry, make proximity labeling a valuable addition to the nematode’s armory.
HostingRepository	PRIDE
AnnounceDate	2021-09-15
AnnouncementXML	Submission_2021-09-15_03:10:27.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Armel Nicolas
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); biotinylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-02 08:24:13	ID requested
⏵ 1	2021-09-15 03:10:28	announced

Artan M, Barratt S, Flynn SM, Begum F, Skehel M, Nicolas A, de Bono M, Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. J Biol Chem, 297(3):101094(2021) [pubmed]

submitter keyword: interactome Caenorhabditis elegans turboID proximity proteomics biotin labeling

Mario de Bono
contact affiliation	IST Austria, Austria
contact email	mario.debono@ist.ac.at
lab head
Armel Nicolas
contact affiliation	IST Austria
contact email	armel.nicolas@ist.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027068

PRIDE project URI

• PRIDE
  1. PXD027068
     1. Label: PRIDE project
     2. Name: Interactome analysis of C. elegans synapses by TurboID-based proximity labeling