<<< Full experiment listing


PXD025630 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleWittig reagents for chemoselective sulfenic acid ligation enables global site stoichiometry analysis and redox-controlled mitochondrial targeting
DescriptionTriphenylphosphonium (TPP) ylides, known as Wittig reagents, are one of the most commonly used tools in synthetic chemistry. Despite their considerable versatility, Wittig reagents have not yet been explored for their utility in biological applications. Here, we introduce a new chemoselective ligation reaction that harnesses the reactivity of Wittig reagents and the unique chemical properties of sulfenic acid, a pivotal post-translational cysteine modification in redox biology. The reaction, which generates a covalent bond between the ylide nucleophilic α-carbon and electrophilic γ-sulfur is highly selective, rapid, and affords robust labeling under a range of biocompatible reaction conditions, including in living cells. We highlight the broad utility of this conjugation method to enable site-specific proteome-wide stoichiometry analysis of S-sulfenylation, visualize redox-dependent changes in mitochondrial cysteine oxidation, and redox-triggered TPP generation for controlled delivery of small molecules to mitochondria.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLing Fu
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList(13)C labeled residue
InstrumentQ Exactive HF-X; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-26 01:05:09ID requested
12021-04-26 01:05:49announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: S-sulfenylation, WyneN
Contact List
Jing Yang
contact affiliationNational Center for Protein Sciences (PHOENIX, Beijing)
contact emailyangjing54@hotmail.com
lab head
Ling Fu
contact affiliationNational Center for Protein Sciences (PHOENIX, Beijing)
contact emailflsmt@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI