PXD025630-1
PXD025630 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Wittig reagents for chemoselective sulfenic acid ligation enables global site stoichiometry analysis and redox-controlled mitochondrial targeting |
| Description | Triphenylphosphonium (TPP) ylides, known as Wittig reagents, are one of the most commonly used tools in synthetic chemistry. Despite their considerable versatility, Wittig reagents have not yet been explored for their utility in biological applications. Here, we introduce a new chemoselective ligation reaction that harnesses the reactivity of Wittig reagents and the unique chemical properties of sulfenic acid, a pivotal post-translational cysteine modification in redox biology. The reaction, which generates a covalent bond between the ylide nucleophilic α-carbon and electrophilic γ-sulfur is highly selective, rapid, and affords robust labeling under a range of biocompatible reaction conditions, including in living cells. We highlight the broad utility of this conjugation method to enable site-specific proteome-wide stoichiometry analysis of S-sulfenylation, visualize redox-dependent changes in mitochondrial cysteine oxidation, and redox-triggered TPP generation for controlled delivery of small molecules to mitochondria. |
| HostingRepository | iProX |
| AnnounceDate | 2021-04-26 |
| AnnouncementXML | Submission_2021-04-26_01:05:49.609.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ling Fu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | (13)C labeled residue |
| Instrument | Q Exactive HF-X; Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-04-26 01:05:09 | ID requested | |
| ⏵ 1 | 2021-04-26 01:05:49 | announced | |
| 2 | 2023-02-09 20:02:28 | announced | 2023-02-10: Update publication information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: S-sulfenylation, WyneN |
Contact List
| Jing Yang | |
|---|---|
| contact affiliation | National Center for Protein Sciences (PHOENIX, Beijing) |
| contact email | yangjing54@hotmail.com |
| lab head | |
| Ling Fu | |
| contact affiliation | National Center for Protein Sciences (PHOENIX, Beijing) |
| contact email | flsmt@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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