PXD024267-2

PXD024267 is an original dataset announced via ProteomeXchange.

Title	The comprehensive stage-specific proteome across human pre-implantation embryo development at single-cell level
Description	Although the landscape of epigenomic and transcriptomic regulation during human pre-implantation development has been depicted by applying single-cell sequencing, the investigation of embryonic proteome is almost unrevealed due to the insufficient input quantity from precious human embryonic samples for traditional mass spectrometry (MS). With applying the state-of-the-art ultrahigh sensitivity mass spectrometry technology and nanoliter-scale oil-air-droplet (OAD) chip, we were able to identify more than three thousand proteins in a single oocytes during human pre-implantation development. Hundreds of stage-specific proteins with significant expressional changes were classified. Many that involve important functions, such as paternal genome reprogramming or DNA methylation, were firstly identified in human embryo studies. We discovered a two peaks of “zygotic proteome activation” (ZPA) at 2-cell and morula stages, in which proteins are mainly associated with epigenetic reprogramming. Our study, for the first time, delineates a comprehensive stage-specific proteome landscape at a single-cell level. It is as well a large step of enriching the panorama of functional genomics across human pre-implantation embryo development.
HostingRepository	iProX
AnnounceDate	2021-04-20
AnnouncementXML	Submission_2023-02-20_18:59:46.674.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuaixin Gao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	time-of-flight

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-19 21:16:43	ID requested
1	2022-11-20 22:40:11	announced
⏵ 2	2023-02-20 18:59:59	announced	2023-02-21: Update information.

Dang Y, Zhu L, Yuan P, Liu Q, Guo Q, Chen X, Gao S, Liu X, Ji S, Yuan Y, Lian Y, Li R, Yan L, Wong CCL, Qiao J, Functional profiling of stage-specific proteome and translational transition across human pre-implantation embryo development at a single-cell resolution. Cell Discov, 9(1):10(2023) [pubmed]

submitter keyword: single-cell, proteome,embryo development

Catherine CL Wong
contact affiliation	Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University
contact email	catherine_wong@bjmu.edu.cn
lab head
Shuaixin Gao
contact affiliation	Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University
contact email	gaoshuaixin@126.com
dataset submitter

iProX dataset URI