PXD024267-1

PXD024267 is an original dataset announced via ProteomeXchange.

Title	The comprehensive stage-specific proteome across human pre-implantation embryo development at single-cell level
Description	Although the landscape of epigenomic and transcriptomic regulation during human pre-implantation development has been depicted by applying single-cell sequencing, the investigation of embryonic proteome is almost unrevealed due to the insufficient input quantity from precious human embryonic samples for traditional mass spectrometry (MS). With applying the state-of-the-art ultrahigh sensitivity mass spectrometry technology and nanoliter-scale oil-air-droplet (OAD) chip, we were able to identify more than three thousand proteins in a single oocytes during human pre-implantation development. Hundreds of stage-specific proteins with significant expressional changes were classified. Many that involve important functions, such as paternal genome reprogramming or DNA methylation, were firstly identified in human embryo studies. We discovered a two peaks of “zygotic proteome activation” (ZPA) at 2-cell and morula stages, in which proteins are mainly associated with epigenetic reprogramming. Our study, for the first time, delineates a comprehensive stage-specific proteome landscape at a single-cell level. It is as well a large step of enriching the panorama of functional genomics across human pre-implantation embryo development.
HostingRepository	iProX
AnnounceDate	2021-04-20
AnnouncementXML	Submission_2022-11-20_22:40:11.134.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuaixin Gao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	time-of-flight

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-19 21:16:43	ID requested
⏵ 1	2022-11-20 22:40:11	announced
2	2023-02-20 18:59:59	announced	2023-02-21: Update information.

Dataset with its publication pending

submitter keyword: single-cell, proteome,embryo development

Catherine CL Wong
contact affiliation	Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University
contact email	catherine_wong@bjmu.edu.cn
lab head
Shuaixin Gao
contact affiliation	Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University
contact email	gaoshuaixin@126.com
dataset submitter

iProX dataset URI