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PXD022586-2

PXD022586 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphorus-Ca2+ signalling in marine diatoms
DescriptionPhosphorus is a critical nutrient controlling phytoplankton growth. Availability of this limiting factor can vary significantly in space and time, particularly in dynamic aquatic ecosystems. Diatoms are important eukaryotic phytoplankton that thrive in regions of pulsed phosphate supply, yet little is known of the sensory mechanisms enabling them to detect and rapidly respond to phosphorus availability. Here we show that phosphorus-starved diatoms utilise a novel Ca2+-dependent signalling pathway to sense and regulate cellular recovery following phosphorus resupply. This pathway, which has not previously been described in eukaryotes, is sensitive to sub-micromolar concentrations of phosphate, alongside a range of environmentally relevant phosphorus forms. Using comparative proteomics, we have characterised early adaptations governing diatom cellular recovery from phosphorus limitation. Strikingly, the dominant response was substantial enhancement of nitrogen assimilation proteins. This led to 12-fold increases in absolute nitrate uptake rates, relative to phosphorus-starved cells. Moreover, we find that the novel phosphorus-Ca2+ signalling pathway controls this primary recovery response. Our findings highlight that fundamental cross-talk between the essential nutrients phosphorus and nitrogen drive diatom recovery from phosphorus limitation. Moreover, a novel Ca2+-dependent phosphorus signalling pathway governs such ecological acclimation responses, and is thus likely critical to the success of diatoms in regions of episodic nutrient supply.
HostingRepositoryPRIDE
AnnounceDate2021-02-22
AnnouncementXMLSubmission_2021-02-22_05:27:57.457.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJoseph Christie-Oleza
SpeciesList scientific name: Phaeodactylum tricornutum CCAP 1055/1; NCBI TaxID: 556484;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-17 06:09:50ID requested
12021-02-22 02:10:20announced
22021-02-22 05:27:58announced2021-02-22: Updated project metadata.
Publication List
10.1016/J.CUB.2020.11.073;
Helliwell KE, Harrison EL, Christie-Oleza JA, Rees AP, Kleiner FH, Gaikwad T, Downe J, Aguilo-Ferretjans MM, Al-Moosawi L, Brownlee C, Wheeler GL, Signaling Pathway Coordinates Environmental Phosphorus Sensing and Nitrogen Metabolism in Marine Diatoms. Curr Biol, ():(2020) [pubmed]
Keyword List
submitter keyword: Ca2+ signalling, marine diatoms, Phaeodactylum tricornutum, phosphorous metabolism, nitrogen metabolism
Contact List
Joseph A. Christie-Oleza
contact affiliationUniversity of the Balearic Islands
contact emailjoseph.christie@uib.eu
lab head
Joseph Christie-Oleza
contact affiliationUniversitat de les Illes Balears
contact emailjoseph.christie@uib.eu
dataset submitter
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PRIDE project URI
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