PXD022586-1

PXD022586 is an original dataset announced via ProteomeXchange.

Title	Phosphorus-Ca2+ signalling in marine diatoms
Description	Phosphorus is a critical nutrient controlling phytoplankton growth. Availability of this limiting factor can vary significantly in space and time, particularly in dynamic aquatic ecosystems. Diatoms are important eukaryotic phytoplankton that thrive in regions of pulsed phosphate supply, yet little is known of the sensory mechanisms enabling them to detect and rapidly respond to phosphorus availability. Here we show that phosphorus-starved diatoms utilise a novel Ca2+-dependent signalling pathway to sense and regulate cellular recovery following phosphorus resupply. This pathway, which has not previously been described in eukaryotes, is sensitive to sub-micromolar concentrations of phosphate, alongside a range of environmentally relevant phosphorus forms. Using comparative proteomics, we have characterised early adaptations governing diatom cellular recovery from phosphorus limitation. Strikingly, the dominant response was substantial enhancement of nitrogen assimilation proteins. This led to 12-fold increases in absolute nitrate uptake rates, relative to phosphorus-starved cells. Moreover, we find that the novel phosphorus-Ca2+ signalling pathway controls this primary recovery response. Our findings highlight that fundamental cross-talk between the essential nutrients phosphorus and nitrogen drive diatom recovery from phosphorus limitation. Moreover, a novel Ca2+-dependent phosphorus signalling pathway governs such ecological acclimation responses, and is thus likely critical to the success of diatoms in regions of episodic nutrient supply.
HostingRepository	PRIDE
AnnounceDate	2021-02-22
AnnouncementXML	Submission_2021-02-22_02:10:20.490.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joseph Christie-Oleza
SpeciesList	scientific name: Phaeodactylum tricornutum CCAP 1055/1; NCBI TaxID: 556484;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-17 06:09:50	ID requested
⏵ 1	2021-02-22 02:10:20	announced
2	2021-02-22 05:27:58	announced	2021-02-22: Updated project metadata.

10.1016/J.CUB.2020.11.073;

submitter keyword: Ca2+ signalling, marine diatoms, Phaeodactylum tricornutum, phosphorous metabolism, nitrogen metabolism

Joseph A. Christie-Oleza
contact affiliation	University of the Balearic Islands
contact email	joseph.christie@uib.eu
lab head
Joseph Christie-Oleza
contact affiliation	Universitat de les Illes Balears
contact email	joseph.christie@uib.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022586

PRIDE project URI

• PRIDE
  1. PXD022586
     1. Label: PRIDE project
     2. Name: Phosphorus-Ca2+ signalling in marine diatoms