PXD022051 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Plastid Proteome of the Nonphotosynthetic Chlorophycean Alga Polytomella parva |
Description | The unicellular, free-living, nonphotosynthetic chlorophycean alga Polytomella parva, closely related to Chlamydomonas reinhardtii and Volvox carteri, contains colorless, starch-storing plastids. The P. parva plastids lack all light-dependent processes but maintain crucial metabolic pathways. The colorless alga also lacks a plastid genome, meaning no transcription or translation should occur inside the organelle. Here, using an algal fraction enriched in plastids as well as publicly available transcriptome data, we provide a proteomic characterization of the P. parva plastid, ultimately identifying several plastid proteins, both by mass spectrometry and bioinformatic analyses. Altogether these results led us to propose a plastid proteome for P. parva, i.e., a set of proteins that participate in carbohydrate metabolism; in the synthesis and degradation of starch, amino acids and lipids; in the biosynthesis of terpenoids and tetrapyrroles; in solute transport and protein translocation; and in redox homeostasis. This is the first detailed plastid proteome from a unicellular, free-living colorless alga. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-10 |
AnnouncementXML | Submission_2020-12-10_01:27:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alfredo Cabrera-Orefice |
SpeciesList | scientific name: Polytomella parva; NCBI TaxID: 51329; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-18 23:36:25 | ID requested | |
⏵ 1 | 2020-12-10 01:27:18 | announced | |
Publication List
Fuentes-Ram, í, rez EO, V, á, zquez-Acevedo M, Cabrera-Orefice A, Guerrero-Castillo S, Gonz, á, lez-Halphen D, The plastid proteome of the nonphotosynthetic chlorophycean alga Polytomella parva. Microbiol Res, 243():126649(2021) [pubmed] |
Keyword List
submitter keyword: Nonphotosynthetic algae, Polytomella parva, Chlorophycean algae, Chlamydomonas reinhardtii, amyloplast, plastid proteome, Q exactive |
Contact List
Dr. Diego González-Halphen |
contact affiliation | Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-243, México 04510, D.F. (Mexico) |
contact email | dhalphen@ifc.unam.mx |
lab head | |
Alfredo Cabrera-Orefice |
contact affiliation | Radboud University Medical Center |
contact email | alfredbiomed@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD022051 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022051
- Label: PRIDE project
- Name: The Plastid Proteome of the Nonphotosynthetic Chlorophycean Alga Polytomella parva