PXD022051

PXD022051 is an original dataset announced via ProteomeXchange.

Title	The Plastid Proteome of the Nonphotosynthetic Chlorophycean Alga Polytomella parva
Description	The unicellular, free-living, nonphotosynthetic chlorophycean alga Polytomella parva, closely related to Chlamydomonas reinhardtii and Volvox carteri, contains colorless, starch-storing plastids. The P. parva plastids lack all light-dependent processes but maintain crucial metabolic pathways. The colorless alga also lacks a plastid genome, meaning no transcription or translation should occur inside the organelle. Here, using an algal fraction enriched in plastids as well as publicly available transcriptome data, we provide a proteomic characterization of the P. parva plastid, ultimately identifying several plastid proteins, both by mass spectrometry and bioinformatic analyses. Altogether these results led us to propose a plastid proteome for P. parva, i.e., a set of proteins that participate in carbohydrate metabolism; in the synthesis and degradation of starch, amino acids and lipids; in the biosynthesis of terpenoids and tetrapyrroles; in solute transport and protein translocation; and in redox homeostasis. This is the first detailed plastid proteome from a unicellular, free-living colorless alga.
HostingRepository	PRIDE
AnnounceDate	2020-12-10
AnnouncementXML	Submission_2020-12-10_01:27:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alfredo Cabrera-Orefice
SpeciesList	scientific name: Polytomella parva; NCBI TaxID: 51329;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-18 23:36:25	ID requested
⏵ 1	2020-12-10 01:27:18	announced

Fuentes-Ram, í, rez EO, V, á, zquez-Acevedo M, Cabrera-Orefice A, Guerrero-Castillo S, Gonz, á, lez-Halphen D, The plastid proteome of the nonphotosynthetic chlorophycean alga Polytomella parva. Microbiol Res, 243():126649(2021) [pubmed]

submitter keyword: Nonphotosynthetic algae, Polytomella parva, Chlorophycean algae, Chlamydomonas reinhardtii, amyloplast, plastid proteome, Q exactive

Dr. Diego González-Halphen
contact affiliation	Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-243, México 04510, D.F. (Mexico)
contact email	dhalphen@ifc.unam.mx
lab head
Alfredo Cabrera-Orefice
contact affiliation	Radboud University Medical Center
contact email	alfredbiomed@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD022051

PRIDE project URI

• PRIDE
  1. PXD022051
     1. Label: PRIDE project
     2. Name: The Plastid Proteome of the Nonphotosynthetic Chlorophycean Alga Polytomella parva