PXD020999 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary |
Description | We describe a method to identify neuropeptides in specific cell-types by the conditional disruption of the gene for carboxypeptidase E (Cpe), an essential neuropeptide-processing enzyme. The loss of Cpe led to the accumulation of neuropeptides containing C-terminal basic residues which are used as tags for affinity-purification followed by identification using quantitative peptidomics. This approach enables the identification of specific forms of neuropeptides in genetically defined populations of cells. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-25 |
AnnouncementXML | Submission_2023-10-25_06:36:58.580.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexandre KeijiTashima |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | methylated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-19 08:32:24 | ID requested | |
1 | 2020-11-20 08:03:11 | announced | |
⏵ 2 | 2023-10-25 06:36:59 | announced | 2023-10-25: Updated project metadata. |
Publication List
Fricker LD, Tashima AK, Fakira AK, Hochgeschwender U, Wetsel WC, Devi LA, Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary. Cell Chem Biol, 28(1):105-112.e4(2021) [pubmed] |
Keyword List
submitter keyword: neuropeptidomics, pituitary, single-cell, brain |
Contact List
Alexandre KeijiTashima |
contact affiliation | Department of Biochemistry, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, SP, Brazil |
contact email | aktashima@unifesp.br |
lab head | |
Alexandre KeijiTashima |
contact affiliation | Universidade Federal de Sao Paulo |
contact email | aktashima@unifesp.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD020999 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020999
- Label: PRIDE project
- Name: Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary