PXD020999-1

PXD020999 is an original dataset announced via ProteomeXchange.

Title	Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary
Description	We describe a method to identify neuropeptides in specific cell-types by the conditional disruption of the gene for carboxypeptidase E (Cpe), an essential neuropeptide-processing enzyme. The loss of Cpe led to the accumulation of neuropeptides containing C-terminal basic residues which are used as tags for affinity-purification followed by identification using quantitative peptidomics. This approach enables the identification of specific forms of neuropeptides in genetically defined populations of cells.
HostingRepository	PRIDE
AnnounceDate	2020-11-20
AnnouncementXML	Submission_2020-11-20_08:03:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Keiji Tashima
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-19 08:32:24	ID requested
⏵ 1	2020-11-20 08:03:11	announced
2	2023-10-25 06:36:59	announced	2023-10-25: Updated project metadata.

Dataset with its publication pending

submitter keyword: neuropeptidomics, single-cell, brain, pituitary

Alexandre Keiji Tashima
contact affiliation	Department of Biochemistry, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, SP, Brazil
contact email	aktashima@unifesp.br
lab head
Alexandre Keiji Tashima
contact affiliation	Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD020999

PRIDE project URI

• PRIDE
  1. PXD020999
     1. Label: PRIDE project
     2. Name: Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary