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PXD020999 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNeuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary
DescriptionWe describe a method to identify neuropeptides in specific cell-types by the conditional disruption of the gene for carboxypeptidase E (Cpe), an essential neuropeptide-processing enzyme. The loss of Cpe led to the accumulation of neuropeptides containing C-terminal basic residues which are used as tags for affinity-purification followed by identification using quantitative peptidomics. This approach enables the identification of specific forms of neuropeptides in genetically defined populations of cells.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexandre Keiji Tashima
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmethylated residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-19 08:32:24ID requested
12020-11-20 08:03:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: neuropeptidomics, single-cell, brain, pituitary
Contact List
Alexandre Keiji Tashima
contact affiliationDepartment of Biochemistry, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, SP, Brazil
contact emailaktashima@unifesp.br
lab head
Alexandre Keiji Tashima
contact affiliationUniversidade Federal de Sao Paulo
contact emailaktashima@unifesp.br
dataset submitter
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