PXD020999

PXD020999 is an original dataset announced via ProteomeXchange.

Title	Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary
Description	We describe a method to identify neuropeptides in specific cell-types by the conditional disruption of the gene for carboxypeptidase E (Cpe), an essential neuropeptide-processing enzyme. The loss of Cpe led to the accumulation of neuropeptides containing C-terminal basic residues which are used as tags for affinity-purification followed by identification using quantitative peptidomics. This approach enables the identification of specific forms of neuropeptides in genetically defined populations of cells.
HostingRepository	PRIDE
AnnounceDate	2023-10-25
AnnouncementXML	Submission_2023-10-25_06:36:58.580.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre KeijiTashima
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-19 08:32:24	ID requested
1	2020-11-20 08:03:11	announced
⏵ 2	2023-10-25 06:36:59	announced	2023-10-25: Updated project metadata.

Fricker LD, Tashima AK, Fakira AK, Hochgeschwender U, Wetsel WC, Devi LA, Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary. Cell Chem Biol, 28(1):105-112.e4(2021) [pubmed]

submitter keyword: neuropeptidomics, pituitary, single-cell, brain

Alexandre KeijiTashima
contact affiliation	Department of Biochemistry, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, SP, Brazil
contact email	aktashima@unifesp.br
lab head
Alexandre KeijiTashima
contact affiliation	Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020999
     1. Label: PRIDE project
     2. Name: Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary