PXD020999 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary |
Description | We describe a method to identify neuropeptides in specific cell-types by the conditional disruption of the gene for carboxypeptidase E (Cpe), an essential neuropeptide-processing enzyme. The loss of Cpe led to the accumulation of neuropeptides containing C-terminal basic residues which are used as tags for affinity-purification followed by identification using quantitative peptidomics. This approach enables the identification of specific forms of neuropeptides in genetically defined populations of cells. |
HostingRepository | PRIDE |
AnnounceDate | 2020-11-20 |
AnnouncementXML | Submission_2020-11-20_08:03:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexandre Keiji Tashima |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | methylated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-19 08:32:24 | ID requested | |
⏵ 1 | 2020-11-20 08:03:11 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: neuropeptidomics, single-cell, brain, pituitary |
Contact List
Alexandre Keiji Tashima |
contact affiliation | Department of Biochemistry, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, SP, Brazil |
contact email | aktashima@unifesp.br |
lab head | |
Alexandre Keiji Tashima |
contact affiliation | Universidade Federal de Sao Paulo |
contact email | aktashima@unifesp.br |
dataset submitter | |
Full Dataset Link List
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020999
- Label: PRIDE project
- Name: Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary