PXD020223 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Small extracellular vesicles-delivered LSD1 promotes gastric cancer cell stemness |
Description | Although there are plenty of researches about nucleic acid in small extracellular vesicles (sEVs), properties of proteins identified as sEVs’ cargos and the mechanism of their action in recipient cell are poorly understood. Here, we show that lysine specific demethylase 1 (LSD1), the first identified histone demethylase in 2004, existed in the cell cultured medium of gastric cancer cells. Further investigation confirmed the presence of LSD1 in sEVs from gastric cancer cells and gastric cancer patient plasma, which is the first identified histone demethylase in sEVs. By shuttling from donor cells to recipient gastric cancer cells, sEVs-delivered LSD1 promoted the cancer cell stemness by positively regulating the expression of Nanog, OCT4, SOX2 and CD44, and suppressed the oxaliplatin response of the recipient cells in vitro and in vivo, while LSD1 depleted sEVs failed to suppress the oxaliplatin response. Collectively, our findings give an evidence for LSD1 as a sEVs protein to promote stemness and suppress oxaliplatin response for the first time and constitute a future avenue to predict oxaliplatin response in gastric cancer clinically. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-05 |
AnnouncementXML | Submission_2021-08-05_02:23:27.926.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Pabst |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-07-06 15:07:23 | ID requested | |
1 | 2021-07-30 11:09:13 | announced | |
⏵ 2 | 2021-08-05 02:23:28 | announced | 2021-08-05: Updated project metadata. |
3 | 2021-12-22 03:28:23 | announced | 2021-12-22: Updated project metadata. |
4 | 2022-03-14 09:47:15 | announced | 2022-03-14: Updated project metadata. |
Publication List
10.1111/1462-2920.15241; |
Zhao LJ, Li YY, Zhang YT, Fan QQ, Ren HM, Zhang C, Mardinoglu A, Chen WC, Pang JR, Shen DD, Wang JW, Zhao LF, Zhang JY, Wang ZY, Zheng YC, Liu HM, Lysine demethylase LSD1 delivered via small extracellular vesicles promotes gastric cancer cell stemness. EMBO Rep, 22(8):e50922(2021) [pubmed] |
Keyword List
submitter keyword: Small extracellular vesicles |
LSD1 |
stemness |
gastric cancer |
Contact List
Dr Martin Pabst |
contact affiliation | Department of Biotechnology, TU Delft, NL |
contact email | m.pabst@tudelft.nl |
lab head | |
Martin Pabst |
contact affiliation | TUD |
contact email | m.pabst@tudelft.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020223
- Label: PRIDE project
- Name: Small extracellular vesicles-delivered LSD1 promotes gastric cancer cell stemness