PXD020223

PXD020223 is an original dataset announced via ProteomeXchange.

Title	Small extracellular vesicles-delivered LSD1 promotes gastric cancer cell stemness
Description	Although there are plenty of researches about nucleic acid in small extracellular vesicles (sEVs), properties of proteins identified as sEVs’ cargos and the mechanism of their action in recipient cell are poorly understood. Here, we show that lysine specific demethylase 1 (LSD1), the first identified histone demethylase in 2004, existed in the cell cultured medium of gastric cancer cells. Further investigation confirmed the presence of LSD1 in sEVs from gastric cancer cells and gastric cancer patient plasma, which is the first identified histone demethylase in sEVs. By shuttling from donor cells to recipient gastric cancer cells, sEVs-delivered LSD1 promoted the cancer cell stemness by positively regulating the expression of Nanog, OCT4, SOX2 and CD44, and suppressed the oxaliplatin response of the recipient cells in vitro and in vivo, while LSD1 depleted sEVs failed to suppress the oxaliplatin response. Collectively, our findings give an evidence for LSD1 as a sEVs protein to promote stemness and suppress oxaliplatin response for the first time and constitute a future avenue to predict oxaliplatin response in gastric cancer clinically.
HostingRepository	PRIDE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-14_09:47:15.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Pabst
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-06 15:07:23	ID requested
1	2021-07-30 11:09:13	announced
2	2021-08-05 02:23:28	announced	2021-08-05: Updated project metadata.
3	2021-12-22 03:28:23	announced	2021-12-22: Updated project metadata.
⏵ 4	2022-03-14 09:47:15	announced	2022-03-14: Updated project metadata.

Sorokin DY, Diender M, Merkel AY, Koenen M, Bale NJ, Pabst M, Sinninghe Damst, é JS, Sousa DZ, Natranaerofaba carboxydovora gen. nov., sp. nov., an extremely haloalkaliphilic CO-utilizing acetogen from a hypersaline soda lake representing a novel deep phylogenetic lineage in the class 'Natranaerobiia'. Environ Microbiol, 23(7):3460-3476(2021) [pubmed]

submitter keyword: Small extracellular vesicles

LSD1

stemness

gastric cancer

Dr Martin Pabst
contact affiliation	Department of Biotechnology, TU Delft, NL
contact email	m.pabst@tudelft.nl
lab head
Martin Pabst
contact affiliation	TUD
contact email	m.pabst@tudelft.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD020223

PRIDE project URI

• PRIDE
  1. PXD020223
     1. Label: PRIDE project
     2. Name: Small extracellular vesicles-delivered LSD1 promotes gastric cancer cell stemness