PXD016633 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mouse Th17 T cells, IL-23 stimulated |
Description | Interleukin 23 (IL-23) triggers pathogenic features in pro-inflammatory, IL-17-secreting T cells (Th17 and Tγδ17) that play a key role in the development of inflammatory diseases. However, the IL-23 signaling cascade remains largely undefined. Here we used quantitative phosphoproteomics to characterize IL-23 signaling in primary murine Th17 cells. We quantified 6,888 phosphorylation sites in Th17 cells, and found 168 phosphorylations regulated upom IL-23 stimulation. IL-23 increased the phosphorylation of the myosin regulatory light chain (RLC), an actomyosin contractibility marker, in Th17 and Tγδ cells. IL-23-induced RLC phosphorylation required JAK2 and ROCK catalytic activity, and the study of the IL-23/ROCK axis revealed an unexpected role of IL-23 in the migration of Tγδ17 and Th17 cells. Moreover, pharmacological inhibition of ROCK reduced Tγδ17 recruitment to inflamed skin upon challenge with inflammatory agent Imiquimod. This work: i) provides new insights into phosphorylation networks that control Th17 cells, ii) widely expands the current knowledge on IL-23 signaling, and iii) contributes to the increasing list of immune cells subsets characterized by global phosphoproteomic approaches. |
HostingRepository | PRIDE |
AnnounceDate | 2020-03-25 |
AnnouncementXML | Submission_2020-03-25_07:13:35.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maria Navarro |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-06 05:22:06 | ID requested | |
⏵ 1 | 2020-03-25 07:13:37 | announced | |
Publication List
Á, lvarez-Salamero C, Castillo-Gonz, á, lez R, Pastor-Fern, á, ndez G, Mariblanca IR, Pino J, Cibrian D, Navarro MN, IL-23 signaling regulation of pro-inflammatory T-cell migration uncovered by phosphoproteomics. PLoS Biol, 18(3):e3000646(2020) [pubmed] |
Keyword List
submitter keyword: Mouse, T lymphocyte, Th17, IL-23 |
Contact List
Maria N. Navarro |
contact affiliation | María N. Navarro Interactions With The Environment Program. Immune System Development and Function Unit. Centro de Biología Molecular "Severo Ochoa" Lab. 126 C/Nicolás Cabrera, 1. 28049 Madrid. Spain Tlf.: (+34) 911964453 email: marian.navarro@cbm.csic.es |
contact email | marian.navarro@cbm.csic.es |
lab head | |
Maria Navarro |
contact affiliation | Centro de Biología Molecular "Severo Ochoa" |
contact email | marian.navarro@cbm.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016633
- Label: PRIDE project
- Name: Mouse Th17 T cells, IL-23 stimulated