PXD014978-2

PXD014978 is an original dataset announced via ProteomeXchange.

Title	Human connective tissue mast cell proteome
Description	Mast cells are hematopoietic cells that reside preferentially in tissues exposed to internal and external environments. Mast cells sense immunological, inflammatory and environmental stimuli, and can be activated to release granules and generate inflammatory mediators. Mast cell-derived products confer protection against snake venoms and some parasite infections. Aberrant activation of mast cells is a major contributor to human pathology, including allergy, asthma and adverse drug reactions. Their strict tissue location has largely impeded the isolation of large numbers of primary mast cell for further analysis. To better understand the biology of mast cells, we analyzed the proteome of primary human and mouse mast cells by quantitative mass spectrometry. We identified a mast cell-specific protein signature that was conserved from mouse to man. Compared to a comprehensive set of other immune cell lineages, proteome analysis identified a unique and distant mast cell cluster. The mast cell signature included proteins governing granule biosynthesis and secretion, as well as proteoglycan- and neurotransmitter metabolism. Proteome conservation across species suggests evolutionary maintenance of mast cell functions.
HostingRepository	PRIDE
AnnounceDate	2020-07-27
AnnouncementXML	Submission_2020-07-27_08:57:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Plum
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-12 01:04:13	ID requested
1	2020-02-12 07:10:42	announced
⏵ 2	2020-07-27 08:57:56	announced	2020-07-27: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Human, fat mast cell, skin mast cell

Hans-Reimer Rodewald
contact affiliation	Department of Cellular Immunology, German Cancer Research Center Heidelberg, Germany
contact email	hr.rodewald@dkfz.de
lab head
Thomas Plum
contact affiliation	German Cancer Reseach Center (DKFZ)
contact email	t.plum@dkfz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD014978

PRIDE project URI

• PRIDE
  1. PXD014978
     1. Label: PRIDE project
     2. Name: Human connective tissue mast cell proteome