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PXD014978 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman connective tissue mast cell proteome
DescriptionMast cells are hematopoietic cells that reside preferentially in tissues exposed to internal and external environments. Mast cells sense immunological, inflammatory and environmental stimuli, and can be activated to release granules and generate inflammatory mediators. Mast cell-derived products confer protection against snake venoms and some parasite infections. Aberrant activation of mast cells is a major contributor to human pathology, including allergy, asthma and adverse drug reactions. Their strict tissue location has largely impeded the isolation of large numbers of primary mast cell for further analysis. To better understand the biology of mast cells, we analyzed the proteome of primary human and mouse mast cells by quantitative mass spectrometry. We identified a mast cell-specific protein signature that was conserved from mouse to man. Compared to a comprehensive set of other immune cell lineages, proteome analysis identified a unique and distant mast cell cluster. The mast cell signature included proteins governing granule biosynthesis and secretion, as well as proteoglycan- and neurotransmitter metabolism. Proteome conservation across species suggests evolutionary maintenance of mast cell functions.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Plum
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-12 01:04:13ID requested
12020-02-12 07:10:42announced
22020-07-27 08:57:56announced2020-07-27: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Human, fat mast cell, skin mast cell
Contact List
Hans-Reimer Rodewald
contact affiliationDepartment of Cellular Immunology, German Cancer Research Center Heidelberg, Germany
contact emailhr.rodewald@dkfz.de
lab head
Thomas Plum
contact affiliationGerman Cancer Reseach Center (DKFZ)
contact emailt.plum@dkfz.de
dataset submitter
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Dataset FTP location
PRIDE project URI
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