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PXD013136 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomic map of the trypanosomatid Strigomonas culicis: the biological contribution of its endosymbiotic bacterium
DescriptionStrigomonas culicis is a kinetoplastid parasite of insects that maintains a mutualistic association with an intracellular symbiotic bacterium, that is highly integrated into the protozoa metabolism: it furnishes essential compounds and divides in synchrony with the nuclear host. The protozoa, conversely, can be rid of the endosymbiont, producing a cured cell line, which presents a diminished ability to colonize the insect host. This obligatory association can represent an intermediate step of the evolution towards the formation of a organelle, therefore representing an interesting model to understand the symbiogenesis theory. Here, we used shotgun proteomics to compare the S. culicis endosymbiont-containing and aposymbiotic strains, revealing a total of 11,305 peptides, and up to 2,213 proteins (2,029 and 1,452 for wild and aposymbiotic, respectively). Gene ontology associated to comparative analysis between both strains revealed that the biological processes most affected by the elimination of the symbiont were the amino acid metabolism, as well as protein synthesis and folding. This large-scale comparison of the protein expression in S. culicis marks a step forward in the comprehension of the role of endosymbiotic bacterium in monoxenic trypanosomatid biology, particularly because these organisms have a polycistronic open reading frame organization and post-transcriptional gene regulation.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRichard Hemmi Valente
SpeciesList scientific name: Strigomonas culicis; NCBI TaxID: 28005;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-19 02:41:15ID requested
12019-11-08 08:57:23announced
22019-11-27 02:16:55announced2019-11-27: Updated publication reference for PubMed record(s): 31760169.
Publication List
Brunoro GVF, Menna-Barreto RFS, Garcia-Gomes AS, Boucinha C, Lima DB, Carvalho PC, Teixeira-Ferreira A, Trugilho MRO, Perales J, Schwämmle V, Catanho M, de Vasconcelos ATR, Motta MCM, d'Avila-Levy CM, Valente RH, Quantitative Proteomic Map of the Trypanosomatid Strigomonas culicis: The Biological Contribution of its Endosymbiotic Bacterium. Protist, 170(6):125698(2019) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: endosymbiont-bearing trypanosomatid
mass spectrometry
amino acid metabolism, protein synthesis, and folding
energetic metabolism
Contact List
Richard Hemmi Valente
contact affiliationLaboratory of Toxinology, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil
contact emailrichardhemmi@gmail.com
lab head
Richard Hemmi Valente
contact affiliationFundação Oswaldo Cruz
contact emailrichardhemmi@gmail.com
dataset submitter
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