PXD013136 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic map of the trypanosomatid Strigomonas culicis: the biological contribution of its endosymbiotic bacterium |
Description | Strigomonas culicis is a kinetoplastid parasite of insects that maintains a mutualistic association with an intracellular symbiotic bacterium, that is highly integrated into the protozoa metabolism: it furnishes essential compounds and divides in synchrony with the nuclear host. The protozoa, conversely, can be rid of the endosymbiont, producing a cured cell line, which presents a diminished ability to colonize the insect host. This obligatory association can represent an intermediate step of the evolution towards the formation of a organelle, therefore representing an interesting model to understand the symbiogenesis theory. Here, we used shotgun proteomics to compare the S. culicis endosymbiont-containing and aposymbiotic strains, revealing a total of 11,305 peptides, and up to 2,213 proteins (2,029 and 1,452 for wild and aposymbiotic, respectively). Gene ontology associated to comparative analysis between both strains revealed that the biological processes most affected by the elimination of the symbiont were the amino acid metabolism, as well as protein synthesis and folding. This large-scale comparison of the protein expression in S. culicis marks a step forward in the comprehension of the role of endosymbiotic bacterium in monoxenic trypanosomatid biology, particularly because these organisms have a polycistronic open reading frame organization and post-transcriptional gene regulation. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-08 |
AnnouncementXML | Submission_2019-11-08_08:57:22.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Richard Hemmi Valente |
SpeciesList | scientific name: Strigomonas culicis; NCBI TaxID: 28005; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-19 02:41:15 | ID requested | |
⏵ 1 | 2019-11-08 08:57:23 | announced | |
2 | 2019-11-27 02:16:55 | announced | 2019-11-27: Updated publication reference for PubMed record(s): 31760169. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: endosymbiont-bearing trypanosomatid |
proteomics |
mass spectrometry |
amino acid metabolism, protein synthesis, and folding |
energetic metabolism |
Contact List
Richard Hemmi Valente |
contact affiliation | Laboratory of Toxinology, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil |
contact email | richardhemmi@gmail.com |
lab head | |
Richard Hemmi Valente |
contact affiliation | Fundação Oswaldo Cruz |
contact email | richardhemmi@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013136
- Label: PRIDE project
- Name: Quantitative proteomic map of the trypanosomatid Strigomonas culicis: the biological contribution of its endosymbiotic bacterium