PXD012849 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of the Zein Protein Composition in Maize Flour Extracts |
Description | Highly homogenous zein protein was isolated from maize kernels in an environment-friendly process using 95 % ethanol as solvent. High purity of the zein protein product was determined by SDS PAGE analysis and by 2 D gel electrophoresis followed by MALDI-ToF-MS peptide mass fingerprinting after in-gel chymotrypsin digestion. Being a natural product that is encoded by multiple gene copies, the polymorphic zein protein product revealed two rows of protein spots, one at 25 kDa and one at 20 kDa apparent molecular mass. MALDI-ToF-MS peptide mapping of the proteins from all spots indicated the presence of only alpha zein proteins. The most prominent ion signals in the MALDI mass spectra after in-gel digestion were recorded at m/z 1083.5 and m/z 1691.8. These ion signals have been found typical for zein proteins and may serve as marker ion signals which upon chymotryptic digestion reliably indicate the presence of zein protein in both hybrid corn products. Due to the given polyploidy and genetic polymorphism of the plant source the application of high resolution separation methods in conjunction with precise analytical methods, such as MALDI-ToF-MS, is required to accurately estimate homogeneity of products that contain natural zein protein. |
HostingRepository | PRIDE |
AnnounceDate | 2019-06-13 |
AnnouncementXML | Submission_2019-06-13_01:43:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Kreutzer |
SpeciesList | scientific name: Zea mays (Maize); NCBI TaxID: 4577; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Bruker Daltonics flex series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-02-25 00:10:45 | ID requested | |
1 | 2019-06-12 06:09:33 | announced | |
⏵ 2 | 2019-06-13 01:43:56 | announced | Updated project metadata. |
3 | 2019-06-13 07:04:46 | announced | Updated project metadata. |
4 | 2019-06-14 01:28:50 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Maize, flour, zein proteins, 2D gel electrophoresis, MALDI-MS, peptide mass fingerprinting, data base search, custom-built sequence data base |
Contact List
Prof. Dr. Michael O. Glocker |
contact affiliation | Proteome Center Rostock Department for Proteome Research Institute of Immunology Medical Faculty and Natural Science Faculty University of Rostock Schillingallee 69 18057 Rostock Germany |
contact email | michael.glocker@med.uni-rostock.de |
lab head | |
Michael Kreutzer |
contact affiliation | University Medical Center Rostock |
contact email | michael.kreutzer@med.uni-rostock.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012849
- Label: PRIDE project
- Name: Characterization of the Zein Protein Composition in Maize Flour Extracts