PXD012849-1

PXD012849 is an original dataset announced via ProteomeXchange.

Title	Characterization of the Zein Protein Composition in Maize Flour Extracts
Description	Highly homogenous ? zein protein was isolated from maize kernels in an environment-friendly process using 95 % ethanol as solvent. High purity of the ? zein protein product was determined by SDS PAGE analysis and by 2 D gel electrophoresis followed by MALDI-ToF-MS peptide mass fingerprinting after in-gel chymotrypsin digestion. Being a natural product that is encoded by multiple gene copies, the polymorphic ? zein protein product revealed two rows of protein spots, one at 25 kDa and one at 20 kDa apparent molecular mass. MALDI-ToF-MS peptide mapping of the proteins from all spots indicated the presence of only ? zein proteins. The most prominent ion signals in the MALDI mass spectra after in-gel digestion were recorded at m/z 1083.5 and m/z 1691.8. These ion signals have been found typical for ? zein proteins and may serve as marker ion signals which upon chymotryptic digestion reliably indicate the presence of ? zein protein in both hybrid corn products. Due to the given polyploidy and genetic polymorphism of the plant source the application of high resolution separation methods in conjunction with precise analytical methods, such as MALDI-ToF-MS, is required to accurately estimate homogeneity of products that contain natural zein protein.
HostingRepository	PRIDE
AnnounceDate	2019-06-12
AnnouncementXML	Submission_2019-06-12_06:09:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics flex series

Revision	Datetime	Status	ChangeLog Entry
0	2019-02-25 00:10:45	ID requested
⏵ 1	2019-06-12 06:09:33	announced
2	2019-06-13 01:43:56	announced	Updated project metadata.
3	2019-06-13 07:04:46	announced	Updated project metadata.
4	2019-06-14 01:28:50	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Maize, flour, zein proteins, 2D gel electrophoresis, MALDI-MS, peptide mass fingerprinting, data base search, custom-built sequence data base

Prof. Dr. Michael O. Glocker
contact affiliation	Proteome Center Rostock Department for Proteome Research Institute of Immunology Medical Faculty and Natural Science Faculty University of Rostock Schillingallee 69 18057 Rostock Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012849
     1. Label: PRIDE project
     2. Name: Characterization of the Zein Protein Composition in Maize Flour Extracts