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PXD012849

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-06-14
  • AnnouncementXML: Submission_2019-06-14_01:28:49.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Michael Kreutzer
  • Title: Characterization of the Zein Protein Composition in Maize Flour Extracts
  • Description: Highly homogenous zein protein was isolated from maize kernels in an environment-friendly process using 95 % ethanol as solvent. High purity of the zein protein product was determined by SDS PAGE analysis and by 2 D gel electrophoresis followed by MALDI-ToF-MS peptide mass fingerprinting after in-gel chymotrypsin digestion. Being a natural product that is encoded by multiple gene copies, the polymorphic zein protein product revealed two rows of protein spots, one at 25 kDa and one at 20 kDa apparent molecular mass. MALDI-ToF-MS peptide mapping of the proteins from all spots indicated the presence of only alpha zein proteins. The most prominent ion signals in the MALDI mass spectra after in-gel digestion were recorded at m/z 1083.5 and m/z 1691.8. These ion signals have been found typical for zein proteins and may serve as marker ion signals which upon chymotryptic digestion reliably indicate the presence of zein protein in both hybrid corn products. Due to the given polyploidy and genetic polymorphism of the plant source the application of high resolution separation methods in conjunction with precise analytical methods, such as MALDI-ToF-MS, is required to accurately estimate homogeneity of products that contain natural zein protein.
  • SpeciesList: scientific name: Zea mays (Maize); NCBI TaxID: 4577;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: Bruker Daltonics flex series

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-02-25 00:10:45ID requested
12019-06-12 06:09:33announced
22019-06-13 01:43:56announcedUpdated project metadata.
32019-06-13 07:04:46announcedUpdated project metadata.
42019-06-14 01:28:50announcedUpdated project metadata.

Publication List

  1. Postu PA, Ion L, Drochioiu G, Petre BA, Glocker MO, Mass spectrometric characterization of the zein protein composition in maize flour extracts upon protein separation by SDS-PAGE and 2D gel electrophoresis. Electrophoresis, ():(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Maize, flour, zein proteins, 2D gel electrophoresis, MALDI-MS, peptide mass fingerprinting, data base search, custom-built sequence data base

Contact List

    Prof. Dr. Michael O. Glocker
    • contact affiliation: Proteome Center Rostock Department for Proteome Research Institute of Immunology Medical Faculty and Natural Science Faculty University of Rostock Schillingallee 69 18057 Rostock Germany
    • contact email: michael.glocker@med.uni-rostock.de
    • lab head:
    Michael Kreutzer
    • contact affiliation: University Medical Center Rostock
    • contact email: michael.kreutzer@med.uni-rostock.de
    • dataset submitter:

Full Dataset Link List

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