PXD011297-2
PXD011297 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Single-point Calibration |
Description | Mass spectrometry (MS) measurements are not inherently calibrated. Researchers use various calibration methods to assign meaning to arbitrary signal intensities and improve precision. Internal calibration (IC) methods use internal standards (IS) such as synthesized or recombinant proteins or peptides to calibrate MS measurements by comparing endogenous analyte signal to the signal from known IS concentrations spiked into the same sample. However, recent work suggests that using IS as IC introduces quantitative biases that affect comparison across studies due to the inability of IS to capture all sources of variation present throughout an MS workflow. Here we describe a single-point external calibration (EC) strategy to calibrate signal intensity measurements to a common reference material, placing MS measurements on the same scale and harmonizing signal intensities between instruments, acquisition methods, and sites. We demonstrate data harmonization between laboratories and methodologies using this generalizable approach. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2019-02-02 |
AnnouncementXML | Submission_2019-02-02_08:28:12.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Lindsay Pino |
SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932; |
ModificationList | Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4) |
Instrument | Q Exactive HF; TSQ Altis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-10-05 12:15:43 | ID requested | |
1 | 2018-10-05 15:01:19 | announced | |
⏵ 2 | 2019-02-02 08:28:14 | announced | Added publication reference |
Publication List
Pino LK, Searle BC, Huang EL, Noble WS, Hoofnagle AN, MacCoss MJ, Calibration Using a Single-Point External Reference Material Harmonizes Quantitative Mass Spectrometry Proteomics Data between Platforms and Laboratories. Anal Chem, 90(21):13112-13117(2018) [pubmed] |
Keyword List
submitter keyword: signal calibration, harmonization, external reference |
Contact List
Michael J MacCoss | |
---|---|
contact affiliation | University of Washington |
contact email | maccoss@uw.edu |
lab head | |
Lindsay Pino | |
contact affiliation | University of Washington |
contact email | lpino@uw.edu |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |