PXD011297-1

PXD011297 is an original dataset announced via ProteomeXchange.

Title	Single-point Calibration
Description	Mass spectrometry (MS) measurements are not inherently calibrated. Researchers use various calibration methods to assign meaning to arbitrary signal intensities and improve precision. Internal calibration (IC) methods use internal standards (IS) such as synthesized or recombinant proteins or peptides to calibrate MS measurements by comparing endogenous analyte signal to the signal from known IS concentrations spiked into the same sample. However, recent work suggests that using IS as IC introduces quantitative biases that affect comparison across studies due to the inability of IS to capture all sources of variation present throughout an MS workflow. Here we describe a single-point external calibration (EC) strategy to calibrate signal intensity measurements to a common reference material, placing MS measurements on the same scale and harmonizing signal intensities between instruments, acquisition methods, and sites. We demonstrate data harmonization between laboratories and methodologies using this generalizable approach.
HostingRepository	PanoramaPublic
AnnounceDate	2018-10-05
AnnouncementXML	Submission_2018-10-05_15:01:19.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lindsay Pino
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive HF; TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-05 12:15:43	ID requested
⏵ 1	2018-10-05 15:01:19	announced
2	2019-02-02 08:28:14	announced	Added publication reference

Dataset with its publication pending

submitter keyword: signal calibration, harmonization, external reference

Michael J MacCoss
contact affiliation	University of Washington
contact email	maccoss@uw.edu
lab head
Lindsay Pino
contact affiliation	University of Washington
contact email	lpino@uw.edu
dataset submitter

Panorama Public dataset URI