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PXD011297 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSingle-point Calibration
DescriptionMass spectrometry (MS) measurements are not inherently calibrated. Researchers use various calibration methods to assign meaning to arbitrary signal intensities and improve precision. Internal calibration (IC) methods use internal standards (IS) such as synthesized or recombinant proteins or peptides to calibrate MS measurements by comparing endogenous analyte signal to the signal from known IS concentrations spiked into the same sample. However, recent work suggests that using IS as IC introduces quantitative biases that affect comparison across studies due to the inability of IS to capture all sources of variation present throughout an MS workflow. Here we describe a single-point external calibration (EC) strategy to calibrate signal intensity measurements to a common reference material, placing MS measurements on the same scale and harmonizing signal intensities between instruments, acquisition methods, and sites. We demonstrate data harmonization between laboratories and methodologies using this generalizable approach.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLindsay Pino
SpeciesList scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationListCarbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive HF; TSQ Altis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-10-05 12:15:43ID requested
12018-10-05 15:01:19announced
22019-02-02 08:28:14announcedAdded publication reference
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: signal calibration, harmonization, external reference
Contact List
Michael J MacCoss
contact affiliationUniversity of Washington
contact emailmaccoss@uw.edu
lab head
Lindsay Pino
contact affiliationUniversity of Washington
contact emaillpino@uw.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI