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PXD011297

DataSet Summary

  • HostingRepository: PanoramaPublic
  • AnnounceDate: 2019-02-02
  • AnnouncementXML: Submission_2019-02-02_08:28:12.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Lindsay Pino
  • Title: Single-point Calibration
  • Description: Mass spectrometry (MS) measurements are not inherently calibrated. Researchers use various calibration methods to assign meaning to arbitrary signal intensities and improve precision. Internal calibration (IC) methods use internal standards (IS) such as synthesized or recombinant proteins or peptides to calibrate MS measurements by comparing endogenous analyte signal to the signal from known IS concentrations spiked into the same sample. However, recent work suggests that using IS as IC introduces quantitative biases that affect comparison across studies due to the inability of IS to capture all sources of variation present throughout an MS workflow. Here we describe a single-point external calibration (EC) strategy to calibrate signal intensity measurements to a common reference material, placing MS measurements on the same scale and harmonizing signal intensities between instruments, acquisition methods, and sites. We demonstrate data harmonization between laboratories and methodologies using this generalizable approach.
  • SpeciesList: scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
  • ModificationList: Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
  • Instrument: Q Exactive HF; TSQ Altis

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-10-05 12:15:43ID requested
12018-10-05 15:01:19announced
22019-02-02 08:28:14announcedAdded publication reference

Publication List

  1. Pino LK, Searle BC, Huang EL, Noble WS, Hoofnagle AN, MacCoss MJ, Calibration Using a Single-Point External Reference Material Harmonizes Quantitative Mass Spectrometry Proteomics Data between Platforms and Laboratories. Anal Chem, 90(21):13112-13117(2018) [pubmed]

Keyword List

  1. submitter keyword: signal calibration, harmonization, external reference

Contact List

    Michael J MacCoss
    • contact affiliation: University of Washington
    • contact email: maccoss@uw.edu
    • lab head:
    Lindsay Pino
    • contact affiliation: University of Washington
    • contact email: lpino@uw.edu
    • dataset submitter:

Full Dataset Link List

  1. Panorama Public dataset URI

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