PXD009785 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LED lamps induce a differential regulation of proteins and enhance somatic embryo maturation in Carica papaya |
Description | The use of light-emitting diode (LED) lamps could be an alternative method to improve somatic embryogenesis, yielding more efficient somatic embryo development and maturation than the use of conventional fluorescent lamps. The aim of this work was to study the influence of light quality on the somatic embryo development and differential abundance of proteins during the maturation of papaya (Carica papaya) cv. ‘Golden’ embryogenic cultures, using LED lamps with different wavelengths. Of all the LED treatments, the white plus medium blue (WmB - 450/530 nm) light resulted in the best somatic embryo production after 28 days of maturation. The WmB and fluorescent treatments generated 82.4 and 47.6 cotyledonary stage somatic embryos, respectively. By a comparative shotgun proteomics analysis between WmB LED and fluorescent lamps treatments, a total of 28 up-regulated and 7 down-regulated proteins were identified. Among the proteins up-regulated in the cultures treated with WmB LED light compared with fluorescent light were the indole-3-acetic acid-amido synthetase (GH3), pyrophosphate-energized vacuolar membrane proton pump (H+-PPase), and actin-depolymerizing factor 2 (ADF2) proteins, which are involved in the regulation of auxin levels by auxin conjugation and transport. Additionally, proteins related to energetic supply, protein metabolism, cell wall remodeling, internal trafficking, and cell division were up-regulated, showing a significantly higher abundance in the embryogenic cultures incubated under WmB LED light than those incubated under fluorescent light. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-11 |
AnnouncementXML | Submission_2019-09-11_10:12:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vanildo Silveira |
SpeciesList | scientific name: Carica papaya; NCBI TaxID: 3649; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-15 03:52:17 | ID requested | |
⏵ 1 | 2019-09-11 10:12:13 | announced | |
Publication List
Almeida FA, Vale EM, Reis RS, Santa-Catarina C, Silveira V, LED lamps enhance somatic embryo maturation in association with the differential accumulation of proteins in the Carica papaya L. 'Golden' embryogenic callus. Plant Physiol Biochem, 143():109-118(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Carica papaya L., Clonal propagation, Comparative proteomics LED, Label-free proteomics, Light-emitting diode, Somatic embryogenesis |
Contact List
Vanildo Silveira |
contact affiliation | Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil. |
contact email | vanildo@uenf.br |
lab head | |
Vanildo Silveira |
contact affiliation | UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE |
contact email | vanildo@uenf.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009785
- Label: PRIDE project
- Name: LED lamps induce a differential regulation of proteins and enhance somatic embryo maturation in Carica papaya