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PXD006482-1

PXD006482 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of Missing Proteins in the Phosphoproteome of Kidney Cancer
DescriptionFinding missing proteins (MPs) has been one of the critical missions of Chromosome-Centric Human Proteome Project (C-HPP) since 2012, twenty-five research teams from seventeen countries have been trying to search adequate and accurate evidence for MPs through various biochemical strategies. In our previous study, we found that phosphoproteomics is one pleasant means to catch low-abundance and membrane proteins which are phosphorylated. Therefore, we speculate it may be an available approach for MPs detection. In this study, kidney cancer and adjacent tissues were used for phosphoproteomics research, we totally identified 8962 proteins including 6415 phosphoproteins, and 44728 phosphosites, of which 10266 were unreported previously. Interestingly, a total of 75 MPs were found, after rigorous screening and manual checking, 26 MPs were ranked as confident MPs by the verification with the synthesized peptides and a stringent manual check of their MS2 spectra, among which 14 MPs were phosphorylated. Functional analysis for 26 MPs revealed that 16 MPs were found to be membrane proteins, 7 MPs were testis-enriched and 1 MPs were kidney-specific. Therefore we concluded that phosphoproteomics is a promising approach in digging MPs for C-HPP studies.
HostingRepositoryPRIDE
AnnounceDate2017-09-01
AnnouncementXMLSubmission_2017-09-01_06:38:56.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD006482
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterFeng Xu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-10 00:44:58ID requested
12017-09-01 06:38:57announced
Publication List
Peng X, Xu F, Liu S, Li S, Huang Q, Chang L, Wang L, Ma X, He F, Xu P, Identification of Missing Proteins in the Phosphoproteome of Kidney Cancer. J Proteome Res, 16(12):4364-4373(2017) [pubmed]
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Kidney Urine (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project
curator keyword: Biomedical
submitter keyword: Chromosome-Centric Human Proteome Project, Missing proteins, phosphoproteomics, kidney cancer, LTQ Orbitrap Velos
Contact List
Ping Xu
contact affiliationNational Center For Protein Sciences ยท Beijing
contact emailxuping@mail.ncpsb.org
lab head
Feng Xu
contact affiliationNCPSB
contact emailxf_future_2005@163.com
dataset submitter
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Dataset FTP location
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