Finding missing proteins (MPs) has been one of the critical missions of Chromosome-Centric Human Proteome Project (C-HPP) since 2012, twenty-five research teams from seventeen countries have been trying to search adequate and accurate evidence for MPs through various biochemical strategies. In our previous study, we found that phosphoproteomics is one pleasant means to catch low-abundance and membrane proteins which are phosphorylated. Therefore, we speculate it may be an available approach for MPs detection. In this study, kidney cancer and adjacent tissues were used for phosphoproteomics research, we totally identified 8962 proteins including 6415 phosphoproteins, and 44728 phosphosites, of which 10266 were unreported previously. Interestingly, a total of 75 MPs were found, after rigorous screening and manual checking, 26 MPs were ranked as confident MPs by the verification with the synthesized peptides and a stringent manual check of their MS2 spectra, among which 14 MPs were phosphorylated. Functional analysis for 26 MPs revealed that 16 MPs were found to be membrane proteins, 7 MPs were testis-enriched and 1 MPs were kidney-specific. Therefore we concluded that phosphoproteomics is a promising approach in digging MPs for C-HPP studies.