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RPXD056882-2

RPXD056882 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary

Title	An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes [Reanalysis: PXD004452]
Description	Here, we present a readily approachable, optimized method for generating deep proteomes. This method takes full advantage of the high resolution that offline HpH fractionation provides and combines it with short gradients and a fast 20 Hz scanning tandem MS (MS/MS) method for subsequent proteome analysis. This combination solves the problems of lower sensitivity usually associated with fast scanning MS/MS methods, and we demonstrate that the concept of running many fractions on short gradients is the sweet-spot in terms of best compromise between instrument time used and sequencing depth obtained. To benchmark this workflow we analyzed the human proteome of the HeLa cervix carcinoma cell line, which is the most commonly used model for studying human cell biology (Masters, 2002).[Original project description]
HostingRepository	jPOST
AnnounceDate	2024-10-30
AnnouncementXML	Submission_2024-10-30_03:00:26.982.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/RPXD056882
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Reanalysis of PXD004452
RepositorySupport	Supported dataset by repository
PrimarySubmitter	jPOST reanalysis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Reanalysis	Revision	Datetime	Status	Title / ChangeLog Entry
	0	2024-10-16 23:08:24	ID requested
0	1	2024-10-30 02:53:33	announced	An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes [Reanalysis: PXD004452]
0	2	2024-10-30 03:00:27	announced	An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes [Reanalysis: PXD004452] 2024-10-30: Updated announce date.

Publication List

no publication

no publication

Keyword List

submitter keyword: reanalysis, high pH reversed-phase fractionation, phosphorylation, acetylation

submitter keyword: reanalysis, high pH reversed-phase fractionation, phosphorylation, acetylation

Contact List

Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliation	jPOST
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003443/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003443/