RPXD049728-1

RPXD049728 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Title	Large-scale Discovery of Substrates of the Human Kinome (PLK3) [Reanalysis: JPST000508]
Description	Kinase -networks are important for cellular signal transduction. Despite tremendous efforts to uncover these signaling pathways, huge numbers of uncharacterized phosphosites still remain in the human proteome. Because of the transient nature of kinase-substrate interactions in vivo, it is almost impossible to identify direct substrates. Here, we present a novel strategy for the rapid and high-throughput discovery for of kinase substrates of kinase using quantitative proteomics. Using 385 purified kinases, we identified a total of 175,574 potential direct kinase substrates of kinases. In addition, we identified novel kinase groups, such as one group containing 30 threonine-directed kinases and another containing 15 serine/threonine/tyrosine kinase sgroup. Surprisingly, we observed that the diversity of substrates for tyrosine kinases was much higher than that for serine-threonine kinases. [Original project description]
HostingRepository	jPOST
AnnounceDate	2024-02-21
AnnouncementXML	Submission_2024-02-21_04:54:44.782.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/RPXD049728
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Reanalysis of JPST000508
RepositorySupport	Supported dataset by repository
PrimarySubmitter	jPOST reanalysis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; alpha-amino dimethylated residue
Instrument	LTQ Orbitrap

Reanalysis	Revision	Datetime	Status	Title / ChangeLog Entry
	0	2024-02-20 01:04:18	ID requested
0	1	2024-02-21 04:54:45	announced	Large-scale Discovery of Substrates of the Human Kinome (PLK3) [Reanalysis: JPST000508]

no publication

submitter keyword: reanalysis, Homo sapiens, HeLa, in vitro kinase assay

Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliation	jPOST
dataset submitter

jPOST dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002717/