RPXD018477 is container for
one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.
Reanalysis Container Summary
| Title | Reanalysis dataset of JPST000204 |
| Description | Quantitative phosphoproteome analyses of colorectal cancer tissues from 24 patients (12 with or without metastasis) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS/MS with iTRAQ labeling(metastasis:iTRAQ115 or 117, no metastasis: iTRAQ114 or 116).
For this reanalysis, MS Datasets were processed for peak picking with MaxQuant v1.6.2.10, and the generated peak lists were searched against human entries of UniProt/Swiss-Prot release 2018_10 using three search engines: MaxQuant v1.6.2.10, X!Tandem v2015.04.01.1, and Comet 2015.02 rev. 0. The decoy database was generated by reversing sequences from the database. Then, the sum of the total number of matched b/y ions and the total number of amino acids comprising the peptide sequence tag were counted to score the reliability of peptide identification independent of these search engines. Finally, the 1% false discovery rate was controlled by the target-decoy approach with this score. |
| HostingRepository | jPOST |
| AnnounceDate | 2020-05-27 |
| AnnouncementXML | Submission_2021-02-04_17:26:21.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/RPXD018477 |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Reanalysis of JPST000204 |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | jPOST reanalysis |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; iTRAQ4plex reporter+balance reagent N-acylated residue; iTRAQ4plex reporter+balance reagent acylated N-terminal; iTRAQ4plex reporter+balance reagent O-acylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Reanalysis | Revision | Datetime | Status | Title / ChangeLog Entry |
|---|
| 0 | 2020-04-12 23:23:15 | ID requested | |
| 0 | 1 | 2020-05-26 23:33:01 | announced | Reanalysis dataset of JPST000204 |
| 0 | 2 | 2021-02-04 17:26:22 | announced | Reanalysis dataset of JPST000204
2021-02-04: Updated description and FTP location. |
| 0 | 3 | 2022-09-18 02:24:53 | announced | Reanalysis dataset of JPST000204
2022-09-18: Updated FTP location. |
Publication List
Keyword List
| submitter keyword: reanalysis |
Contact List
| Yasushi Ishihama |
|---|
| lab head | |
| jPOST reanalysis |
|---|
| contact affiliation | jPOST |
| contact email | reanalysis@jpostdb.org |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000803 |
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