PXD083970-1

PXD083970 is an original dataset announced via ProteomeXchange.

Title	ATP5D-mediated rejuvenation of peripheral B cells alleviates Alzheimer's disease-like pathologies via mitochondrial transfer
Description	This dataset contains 4D‑DIA quantitative proteomic data from mouse splenic B cells and B‑cell‑conditioned medium (BCM). B‑cells were isolated from young (2‑4 months), old (12‑15 months), AD model (12‑15 months), ATP5D KO (12‑15 months), ATP5D KI (12‑15 months), AD+ATP5D KO (12‑15 months), and AD+ATP5D KI (12‑15 months) mice. BCM samples were collected from young and old mouse B‑cell cultures. Sample preparation including protein extraction, reduction, alkylation, trypsin digestion and desalting was performed. LC‑MS/MS acquisition was carried out on an Orbitrap Q Exactive Plus mass spectrometer. Raw MS files and quantitative proteomics results are deposited in this iProX project. Database searching was performed against the UniProt database for Mus musculus using MaxQuant.
HostingRepository	iProX
AnnounceDate	2026-09-10
AnnouncementXML	Submission_2026-09-10_02:21:29.810.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongmin Niu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive HF-X; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-10 02:21:02	ID requested
⏵ 1	2026-09-10 02:21:30	announced

Dataset with its publication pending

submitter keyword: mouse splenic B cell, B‑cell‑conditioned medium, BCM, 4D‑DIA, LC‑MS/MS, quantitative proteomics, ATP5D

Tinghua Wang
contact affiliation	West China Hospital, Sichuan University
contact email	tinghua9988@wchscu.edu.cn
lab head
Yongmin Niu
contact affiliation	Institute of Neuroscience, Kunming Medical University
contact email	421089982@qq.com
dataset submitter

iProX dataset URI