PXD083934-1

PXD083934 is an original dataset announced via ProteomeXchange.

Title	Synthetic genome expansion reveals a transcriptional constraint on genome size
Description	Genome sizes vary across eukaryotes, largely because of differences in non-coding DNA. However, the physiological effects of this excess DNA are unclear. We engineered budding yeast strains carrying up to 12.8 Mb of predominantly non-coding human DNA, doubling the genome without altering endogenous genes. Genome expansion slowed growth and increased cell size. Spike-in-normalized ChIP-seq and RNA-seq showed that added DNA recruited RNA polymerase II, diverting it from endogenous genes and lowering endogenous mRNA concentration. Ribosome profiling and proteomics revealed little translation from added sequences. A model linked this transcriptional competition to reduced ribosome activity and growth. Thus, excess DNA imposes a fitness cost by sequestering transcriptional resources, providing a mechanistic basis for selection against genome expansion in rapidly proliferating organisms such as yeasts and bacteria.
HostingRepository	PRIDE
AnnounceDate	2026-09-10
AnnouncementXML	Submission_2026-09-09_19:41:04.476.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Lanz
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-09 18:40:11	ID requested
⏵ 1	2026-09-09 19:41:05	announced

Dataset with its publication pending

submitter keyword: synthetic biology, YACs,non-coding DNA

Jan Skotheim
contact affiliation	Department of Biology
contact email	skotheim@stanford.edu
lab head
Michael Lanz
contact affiliation	Stanford University
contact email	mikelanz@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD083934

PRIDE project URI

• PRIDE
  1. PXD083934
     1. Label: PRIDE project
     2. Name: Synthetic genome expansion reveals a transcriptional constraint on genome size