PXD083640-1

PXD083640 is an original dataset announced via ProteomeXchange.

Title	The piR-016176-PIWIL1-SAP18 regulatory network links tumor suppression to acetylation remodeling in pancreatic ductal adenocarcinoma (acetylation)
Description	PIWI-interacting RNAs (piRNAs) are increasingly implicated in cancer, but their regulatory roles in pancreatic ductal adenocarcinoma (PDAC) remain poorly defined. Here, piRNA expression profiling of 10 paired PDAC and adjacent tissues identified piR-016176 as a markedly downregulated candidate, which was subsequently validated in an independent cohort of 50 paired tissues and PDAC cell lines. Gain- and loss-of-function experiments showed that piR-016176 suppressed cell viability, migration, and invasion while promoting apoptosis. DIA quantitative proteomics identified SAP18 as a reciprocally regulated downstream candidate, and bidirectional rescue experiments supported SAP18 as a functional mediator of piR-016176 activity. Computational prediction and RIP-qPCR further demonstrated that piR-016176 and SAP18 mRNA were enriched in PIWIL1-associated ribonucleoprotein complexes. Stable SAP18 depletion induced extensive acetylation-site remodeling, with 181 increased and 4 decreased sites, involving proteins associated with RNA regulation and mitochondrial metabolism. Integrated RNA-seq and DIA proteomic analyses following piR-016176 overexpression identified 112 concordantly upregulated targets enriched in interferon- and immune-related programs. Collectively, these findings identify piR-016176 as a tumor-suppressive piRNA in PDAC and establish SAP18 as a functional downstream effector, while implicating PIWIL1-associated regulation, acetylation remodeling, and interferon-related molecular programs as components of a broader regulatory framework requiring further mechanistic validation.
HostingRepository	iProX
AnnounceDate	2026-09-03
AnnouncementXML	Submission_2026-09-03_18:13:05.950.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaotong Cui
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-03 18:11:59	ID requested
⏵ 1	2026-09-03 18:13:06	announced

Dataset with its publication pending

submitter keyword: Pancreatic ductal adenocarcinoma, piR-016176，PIWIL1, SAP18, protein acetylation, interferon signaling

Zusen Wang
contact affiliation	the Affilliated Hospital of Qingdao University
contact email	wangzusen@126.com
lab head
Xiaotong Cui
contact affiliation	the Affilliated Hospital of Qingdao University
contact email	cxt0079@163.com
dataset submitter

iProX dataset URI