PXD083320-1

PXD083320 is an original dataset announced via ProteomeXchange.

Title	Motile Vesicles or MoVes are a migration-associated extracellular vesicle population carrying genomic DNA
Description	Migrating cancer cells release diverse extracellular vesicles (EVs) that carry bioactive cargo capable of influencing tumor progression and intercellular communication. However, the mechanisms by which genomic DNA and other intracellular components are incorporated into EVs during migration remain poorly understood. Here, we identify and characterize a previously undescribed population of large EVs (2–5 μm), termed Motile Vesicles (MoVes), that are generated during active cancer cell migration and associated with epithelial–mesenchymal transition (EMT). Live-cell imaging and scanning electron microscopy reveal that MoVes arise from trailing-edge retraction during mesenchymal migration and are actively shed by cancer cells but not by non-transformed cells. Whole-genome sequencing demonstrates that MoVes contain genomic DNA spanning the genome of the parent cell, recapitulating its mutational landscape. Comparative analyses across EV populations show that MoVes display distinct ultrastructural features, molecular composition, and sedimentation behavior. Proteomic profiling identifies enrichment of proteins associated with EMT and organelle biology, including the EMT regulator FAM3C, and ultrastructural analyses reveal incorporation of intracellular organelles such as mitochondria. Functional assays further demonstrate that MoVes-associated mitochondrial cargo can enhance oxidative phosphorylation in recipient cells. Together, these findings identify MoVes as a migration-associated EV population that carries genomic DNA and organelle cargo during cancer cell migration, revealing a previously unrecognized mechanism of extracellular DNA and organelle dissemination in cancer.
HostingRepository	PRIDE
AnnounceDate	2026-08-28
AnnouncementXML	Submission_2026-08-28_14:49:30.980.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thupten Tsering
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-27 23:47:10	ID requested
⏵ 1	2026-08-28 14:49:31	announced

Dataset with its publication pending

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: epithelial-mesenchymal transition, FAM3C,Extracellular vesicles, migration, extracellular vesicle-associated DNA, WGS, Motile Vesicles

Julia Burnier
contact affiliation	1Research Institute of the McGill University Health Centre, Montreal, QC, Canada 2Department of Pathology, McGill University, Montreal, QC, Canada 3Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada
contact email	julia.burnier@mcgill.ca
lab head
Thupten Tsering
contact affiliation	RI-MUHC
contact email	thupten.tsering@mail.mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD083320

PRIDE project URI

• PRIDE
  1. PXD083320
     1. Label: PRIDE project
     2. Name: Motile Vesicles or MoVes are a migration-associated extracellular vesicle population carrying genomic DNA