PXD083177-1

PXD083177 is an original dataset announced via ProteomeXchange.

Title	Chemically Grafted PEGylation Mitigates Opsonin Adsorption and Enhances Tumor Targeting of MMP-9-Responsive Nanoparticles
Description	Active-targeting nanocarriers are promising for precision cancer therapy, but protein corona formation upon blood contact masks targeting ligands and triggers rapid clearance. Here, we investigate how PEG surface architecture (chemical grafting vs. physical coating) modulates protein corona formation and therapeutic outcomes. Using a star-shaped polymer (PET-PCL) functionalized with an MMP-9-targeted and responsive dual-functional peptide, we engineered two types of nanoparticles (NPs) with similar bulk properties but different surface poly(ethylene glycol) (PEG) configurations: chemical grafting versus physical coating. Quantitative proteomic analyses revealed that serum incubation led to markedly different protein corona profiles. Notably, chemically grafted NPs exhibited significantly lower adsorption of corona proteins, effectively mitigating opsonin-mediated clearance and preserving targeting functionality. Consequently, these corona-coated NPs demonstrated superior cellular internalization in A549 lung cancer cells and reduced phagocytosis by RAW264.7 macrophages compared with their physically coated counterparts. Furthermore, in vivo biodistribution studies in A549 tumor-bearing mice confirmed that chemically grafted NPs achieved enhanced tumor accumulation, which corresponded with favorable antitumor activity. Collectively, these findings elucidate the critical role of surface PEG architecture in regulating protein corona formation and demonstrate that tailored surface engineering can harmonize stealth and targeting functionalities. This work provides a rational design strategy for next-generation targeted nanomedicines aimed at overcoming biological barriers in complex physiological environments.
HostingRepository	iProX
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-25_18:38:08.639.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haoyu Zhang
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-25 18:37:42	ID requested
⏵ 1	2026-08-25 18:38:09	announced

Dataset with its publication pending

submitter keyword: protein corona,PEGylation,nanoparticles,MMP-9 responsive

Fangyuan Guo
contact affiliation	Zhejiang University of Technology
contact email	guofy@zjut.edu.cn
lab head
Haoyu Zhang
contact affiliation	Zhejiang University of Technology
contact email	a15755475428@163.com
dataset submitter

iProX dataset URI