PXD083139-1

PXD083139 is an original dataset announced via ProteomeXchange.

Title	Investigating N-terminal shedding of GPR125 in GPR125 overexpressing HEK293T cells using N- and C-terminal enrichment and different cleavage strategies
Description	The study was conducted to elucidate potential cleavage sites of GPR125 which are known to undergo N-terminal shedding through autoproteolysis in a region different to other GRPs. To do these different enzymatic strategies as well as enrichment of both N-terminal and C-terminal peptides, was conducted on media from GPR125 overexpressing HEK cells. Other sample types such as media from non-transfected HEK cells and non-PNGnase treated media showed barely any signal from GPR125. GluC was also used as the common clevage site is located in a region devoid of R/K residues. However, few GPR125 originating peptides were identified from these samples compared to Trypsin and TrypN treated samples. Lysate was also briefly analysed resulting in peptides spanning more of the C-terminal end of the protein which were not previously found in the up-concentrated media.
HostingRepository	PRIDE
AnnounceDate	2026-08-31
AnnouncementXML	Submission_2026-08-31_04:02:41.666.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicoline Wichmand Thorsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-25 08:54:35	ID requested
⏵ 1	2026-08-31 04:02:42	announced

Dataset with its publication pending

submitter keyword: GPR125, SCX, C-terminal, Lysate, HEK293T, enrichment,LC-MSMS, ADRA3, N-terminal, Medium

Michael J Davies
contact affiliation	University of Copenhagen, Faculty of Health and Medical Sciences, Department of Biomedical Sciences
contact email	Davies@sund.ku.dk
lab head
Nicoline Wichmand Thorsen
contact affiliation	University of Copenhagen
contact email	nicoline@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD083139
     1. Label: PRIDE project
     2. Name: Investigating N-terminal shedding of GPR125 in GPR125 overexpressing HEK293T cells using N- and C-terminal enrichment and different cleavage strategies