PXD083089-1

PXD083089 is an original dataset announced via ProteomeXchange.

Title	Design and Breeding of High-Yield, Pine Wilt-Resistant Masson Pine Varieties
Description	Proteomic profiling was performed by nano liquid chromatography–tandem mass spectrometry (nanoLC–MS/MS) operated in data‑independent acquisition (DIA) mode. For retention‑time calibration and quantitative quality control, iRT peptides were spiked into each sample prior to injection at an iRT:sample ratio of 1:20 (v/v). Peptides were separated on a nanoElute nanoLC system (Bruker) equipped with a C18 analytical column (25 cm × 75 μm i.d., 1.6 μm C18, IonOpticks) at a flow rate of 300 nL/min, using mobile phase A (0.1% formic acid in water) and mobile phase B (0.1% formic acid in acetonitrile). The DIA gradient was: 0 min, 2% B; 45 min, 22% B; 50 min, 37% B; 55–60 min, 80% B (followed by re‑equilibration to the initial conditions as defined in the instrument method). MS data were acquired on a timsTOF Pro 2 high‑resolution mass spectrometer (Bruker) in DIA mode. The acquisition parameters were: capillary voltage 1.4 kV; dry temperature 180°C; dry gas 3.0 L/min; mass range m/z 100–1700; ion mobility 0.7–1.3 (as defined in the acquisition method); collision energy 20–59 eV. DIA raw files were processed and quantified using Spectronaut Pulsar (v17.5, Biognosys) with the protein sequence database uniprot-Pinus subgen. Pinus-139271-2023.7.3.fasta, and quantitative reports were exported for downstream analysis.
HostingRepository	iProX
AnnounceDate	2026-08-20
AnnouncementXML	Submission_2026-08-24_18:22:57.228.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min Li
SpeciesList	scientific name: Pinus massoniana; NCBI TaxID: 88730;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-24 18:22:37	ID requested
⏵ 1	2026-08-24 18:22:57	announced

Dataset with its publication pending

submitter keyword: Pinus, proteomic, LC-MS/MS

Min Li
contact affiliation	Nanjing Forestry University
contact email	lm950330@njfu.edu.cn
lab head
Min Li
contact affiliation	Nanjing Forestry University
contact email	lm950330@njfu.edu.cn
dataset submitter

iProX dataset URI