PXD083044-1

PXD083044 is an original dataset announced via ProteomeXchange.

Title	Clinical Amyloid Subtyping from FFPE Biopsies Using a Rapid Preparation Workflow and High-Flow LC-Q Exactive MS
Description	This dataset contains mass spectrometry-based proteomics data generated for the clinical identification and subtyping of amyloid deposits from formalin-fixed, paraffin-embedded (FFPE) tissue biopsies. The study evaluates a rapid sample preparation workflow coupled with high-flow liquid chromatography–tandem mass spectrometry (LC-MS/MS) for the characterization of amyloid-associated proteins. FFPE tissue sections were processed using Congo red staining and targeted microdissection of amyloid-positive regions. Proteins were extracted using a heat-assisted extraction procedure followed by rapid tryptic digestion. The resulting peptide mixtures were analyzed using high-flow liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer operated in data-dependent acquisition mode. The dataset includes representative proteomic data generated from clinical amyloid tissue specimens. Protein identification and characterization were performed based on peptide-spectrum matches, unique peptide identification, sequence coverage, and detection of amyloid-associated proteins. Amyloid subtype assignment was supported by the identification of a predominant amyloidogenic protein together with additional amyloid-associated proteins. The dataset is intended to support the evaluation and reproducibility of mass spectrometry-based amyloid identification and subtyping from FFPE clinical tissue specimens. All submitted data have been de-identified, and patient-identifying information has been removed from the dataset.
HostingRepository	iProX
AnnounceDate	2026-08-20
AnnouncementXML	Submission_2026-08-24_02:01:05.029.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naga veera Yerra
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-24 02:00:41	ID requested
⏵ 1	2026-08-24 02:01:05	announced

Dataset with its publication pending

submitter keyword: Amyloidosis, amyloid subtyping, FFPE tissue, clinical proteomics, LC-MS/MS, mass spectrometry, Q Exactive Orbitrap, high-flow liquid chromatography, Congo red, protein identification, amyloid-associated proteins

Naga veera Yerra
contact affiliation	The Mount sinai Hospital
contact email	nagaveeray@gmail.com
lab head
Naga veera Yerra
contact affiliation	The Mount sinai Hospital
contact email	nagaveeray@gmail.com
dataset submitter

iProX dataset URI