PXD083032-1

PXD083032 is an original dataset announced via ProteomeXchange.

Title	surface proteome of WT vs. MGAT1 KO HeLa cells
Description	Using cell-surface biotinylation followed by mass spectrometry (MS), we revealed substantial differences between the surface proteomes of WT and MGAT1 KO HeLa cells. Among the most strongly depleted proteins in MGAT1 KO cells were those involved in cell adhesion and extracellular interactions, membrane domain organization, and cytoskeletal organization. These include LGALS3 (galectin-3) that cross-links complex N-glycans thus stabilizes these glycoproteins at the surface27; ITGA4, ITGA7 (integrin- α4 and α7), matrix adhesion receptors that also contribute to raft stabilization through their clustering and scaffolding; ICAM1, MCAM, and BCAM that mediate cell-cell and cell-matrix interactions; and CAVIN1, the caveolae-associated scaffolding protein that is essential, together with LGALS3, for caveolae biogenesis. These observations suggest that MGAT1 loss broadly compromises the integrity of host cell membrane microdomain.
HostingRepository	PRIDE
AnnounceDate	2026-09-05
AnnouncementXML	Submission_2026-09-05_11:24:38.534.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Klil Cohen Yaron
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-23 10:06:26	ID requested
⏵ 1	2026-09-05 11:24:39	announced

Dataset with its publication pending

submitter keyword: surface proteome, MGAT1

Ilan Rosenshine
contact affiliation	1Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalem, Israel.
contact email	ilanr@ekmd.huji.ac.il
lab head
Klil Cohen Yaron
contact affiliation	Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalem.
contact email	klil.cohen@mail.huji.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD083032

PRIDE project URI

• PRIDE
  1. PXD083032
     1. Label: PRIDE project
     2. Name: surface proteome of WT vs. MGAT1 KO HeLa cells