PXD082661-1

PXD082661 is an original dataset announced via ProteomeXchange.

Title	DIA-based quantitative proteomic analysis of U87 glioblastoma cells following optogenetic CRAC channel activation (LOV2-STIM1 + blue light stimulation)
Description	Glioblastoma (GBM) is the most aggressive primary brain tumor. This dataset contains label-free quantitative proteomic profiles of U87 human glioblastoma cells transduced with the optogenetic construct LOV2-STIM1-mCherry, comparing cells kept in the dark (CTL, n = 3 biological replicates) with cells subjected to 470 nm blue light stimulation (G, n = 3 biological replicates), acquired on a Bruker timsTOF mass spectrometer in data-independent acquisition (DIA) mode. The data support the finding that optogenetic CRAC channel activation induces NLRP3/caspase-1/GSDMD-mediated pyroptosis and inflammatory signaling in GBM cells, as described in the associated manuscript. Proteins with a fold change > 2 and P < 0.05 were considered differentially expressed and subjected to Gene Ontology enrichment analysis.
HostingRepository	PRIDE
AnnounceDate	2026-08-23
AnnouncementXML	Submission_2026-08-23_01:00:11.605.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongli Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-16 08:10:25	ID requested
⏵ 1	2026-08-23 01:00:12	announced

Dataset with its publication pending

submitter keyword: glioblastoma, DIA, label-free quantitative proteomics, optogenetics, CRAC channel, pyroptosis, calcium signaling, timsTOF, U87, LOV2-STIM1

Hongli Li
contact affiliation	Teaching laboratory center, Third Military Medical University, Chongqing, China.
contact email	lihongli@tmmu.edu.cn
lab head
Hongli Li
contact affiliation	Third Military Medical University (Army Medical University), China, Chongqing
contact email	lihongli@tmmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD082661
     1. Label: PRIDE project
     2. Name: DIA-based quantitative proteomic analysis of U87 glioblastoma cells following optogenetic CRAC channel activation (LOV2-STIM1 + blue light stimulation)